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短时受精结合早期补救卵胞浆内单精子注射在IVF-ET治疗中的应用 被引量：2

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摘要 目的观察短时受精结合早期补救卵胞浆内单精子注射(ICSI)能否改善辅助生殖治疗的结局。方法对常规体外受精-胚胎移植(IVF-ET)治疗周期,精卵共培养4h后脱颗粒观察,如排出第二极体的卵母细胞数目少于总MⅡ期卵母细胞数目的 30%以下或全部未见第二极体者,再继续观察2个小时,如果仍然没有改变,实施早期补救ICSI。结果 226个IVF周期中有17个周期实施了早期补救ICSI,补救率为7.52%(17/226),早期补救ICSI新鲜胚胎移植周期临床妊娠率为46.15%(6/13);同时进行的85个常规ICSI周期的新鲜胚胎移植临床妊娠率为44.0%(33/75)。结论短时受精结合早期补救ICSI可避免完全受精失败和低受精率,临床妊娠率同常规ICSI没有差别,可有效减少周期取消率,改善辅助生殖治疗的结局,是值得采用的方法。
作者 王喜良 周敏 白雪 肖玉红 闫素文
机构地区 解放军第
出处 《中国优生与遗传杂志》 2011年第6期104-104,103,共2页 Chinese Journal of Birth Health & Heredity
关键词 短时受精 卵胞浆内单精子注射 早期补救ICSI 体外受精 胚胎移植
分类号 R714.8 [医药卫生—妇产科学]
  • 相关文献

参考文献5

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二级参考文献19

  • 1李晓红,王嵩,张晓,武学清,郭应禄.短时受精与卵胞浆内单精子注射的临床应用研究[J].中华泌尿外科杂志,2004,25(7):485-487. 被引量：7
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  • 3柴三明,刘珍,倪亚莉,王琰.体外受精中精卵孵育时间对结局的影响[J].中国优生与遗传杂志,2006,14(10):83-84. 被引量：7
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共引文献18

同被引文献15

  • 1徐鸿毅,罗清炳,董毅飞,陈娟,江胜芳,张鑫,张昌军.缩短精子孵育时间对体外受精-胚胎移植结局的影响[J].湖北医药学院学报,2012,31(1):1-3. 被引量：6
  • 2庄广伦.现代辅助生殖技术.北京:人民卫生出版社,2005:140-142.
  • 3Menezo Y, Barak Y. Comparison between day-2 embryos obtained either from ICSI or resulting from short insemination IVF: influence of maternal age. Hum Reprod 2000; 15 (8): 1776-1780.
  • 4O'Flaherty C, de Lamirande E, Gagnon C. Positive role of reactive oxygen species in mammalian sperm capacitation: triggering and modulation of phosphorylation events. Free Radic BiolMed 2006, 41(4): 528-540.
  • 5DAlessandro AG, Martemucci G.Taibi L. How the FSH/LH ratio and dose numbers in the p-FSH administration treatment regimen, and insemination schedule affect superovulatory response in ewes. Theriogenology 2005; 63(6): 1764-1774.
  • 6Bungum M, Humaidan P, Axmon A, et al. Sperm DNA integrity assessment in prediction of assisted reproduction technology outcome.HumReprod 2007; 22(1): 174-179.
  • 7Dirnfeld M, Bider D, Koifman M, etal. Shortened exposure of oocytes to spermatozoa improves in-vitro fertilization outcome: a prospective, randomized, controlled study. Hum Reprod 1999; 14 (10): 2562-2564.
  • 8Enkhmaa D, Kasai T, Hoshi K. Long-time exposure of mouse embryos to the sperm produces high levels of reactive oxygen species in culture medium and relates to poor embryo development. Reprod DomestAnim 2009; 44(4): 634-637.
  • 9Barraud-Lange V, Sifer C, Pocate K, eta1. Short gamete co-incubation during in vitro fertilization decreases the fertilization rate and does not improve embryo quality a prospective auto controlled study. JAssist Reprod Genet 2008, 25(7): 305-310.
  • 10方伟芬,冯贵雪,张波,周红,刘茵.精卵孵育时间对胚胎质量的影响[J].广西医学,2008,30(9):1333-1334. 被引量：18

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中国优生与遗传杂志
2011年 第6期

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