摘要
目的:探讨北寄生提取物对人肝癌HepG2细胞增殖抑制和凋亡诱导作用。方法:分别采用噻唑蓝(MTT)法检测细胞增殖活性、光镜观察细胞结构变化、流式细胞仪检测细胞凋亡。结果:0.3mg.mL-1北寄生提取物处理24h后,细胞增殖明显受到抑制,且抑制率随时间延长而增加;光镜下可观察到细胞凋亡形态的改变;0.3mg.mL-1北寄生提取物处理48h后,流式细胞仪检测细胞凋亡率为24.7%。结论:北寄生提取物不仅能有效地抑制肿瘤细胞的增殖,同时又有诱导肿瘤细胞凋亡的作用。
Objective:To explore the anti-proliferation and apoptosis-induced effects of Viscum coloratum extracts(VCE) on HepG2 cells.Methods:HepG2 cell proliferative activity was measured by MTT assay.Cell apoptosis was observed by Giemsa staining method.Cell apoptosis rate was determined by flow cytometry.Results:After exposure to VCE at concentrations 0.3mg·mL-1 for 24h,cellular proliferation was inhibited with the increase of the concentration and the time.The significant apoptotic morphology changes were observed.After exposure to VCE at concentrations 0.3mg·mL-1 for 48h,cell apoptosis was determined as 24.7%.Conclusion:VCE can not only inhibit the proliferation,but also induce apoptosis in HepG2 cells.
出处
《中医药信息》
2011年第3期56-57,共2页
Information on Traditional Chinese Medicine
基金
黑龙江省教育厅科学技术研究项目(11521261)
黑龙江省青年科学技术专项基金资助项目(QC08C78)
黑龙江中医药大学研究生创新基金资助项目(YJSCX2008-143HLJ)