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广东地区猪源戊型肝炎病毒的检测和核苷酸序列分析 被引量:2

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摘要 为了检测广东地区部分猪场猪群中的猪源戊型肝炎病毒(HEV)RNA,试验利用反转录巢式聚合酶链式反应(RT-nPCR)并应用生物学软件将经扩增和测序得到的猪源HEV核苷酸序列与GenBank上的各个基因型HEV进行相似性比较分析。结果表明:5个样品中检测到HEV RNA,它们的相似性为85.1%~96.3%,与GenBank上基因Ⅰ型、Ⅱ型、Ⅲ型和Ⅳ型HEV序列的相似性分别为76.1%~80.5%、76.5%~79.4%、76.4%~80.2%、82.1%~97.7%。说明广东地区存在的猪源HEV可能属于在国内猪群中流行的基因Ⅳ型。
出处 《黑龙江畜牧兽医》 CAS 北大核心 2011年第5期94-96,共3页 Heilongjiang Animal Science And veterinary Medicine
基金 国家生猪现代农业产业技术体系(NYCYTX009)
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参考文献6

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