科学级冷却型CCD-荧光图像系统定量性能的鉴定

IDENTIFICATION ABOUT THE QUANTITATIVE FUNCTION OF THE SCIENTIFIC COOLED CCD-FLUORESCENCE IMAGES SYSTEM

以荧光探针Hoechst33342（Ho3334）特异性标记大白鼠精子和肝细胞DNA，用科学级冷却型CCD显微荧光图像系统获取单个细胞的荧光图像，再以图像处理软件“Image-ProPlus”，分析测量各细胞的DNA相对含量和细胞核的相对面积．实验数据表明：鼠肝细胞的DNA相对含量分别为精子的DNA相对含量的2，4，8倍，符合生物学的规律，说明这套系统的定量结果是可靠的．

The DNA of rat spermatzoa and hepatocytes was stained with specifical fluoeescence probe Hoechst33342. The fluoerecence of single cell were archived by the scientific cooled CCD- microscopy fluorescence images spstem DNA relative content and area were analyzed software. The results show that the DNA relative content of rat hepatocytes are 2, 4, 8 times of that of rat spermatozoa respetively, which confoams to the biological law.It indicates that the quantitative result of the system used is reliable.