全细胞生物催化竹笋壳制备燃料乙醇的研究

STUDY ON PRODUCING FUEL-ETHANOL FROM BAMBOO SHOOTS SHELL FIBER(BSSF) CATALIZED BY WHOLE CELL BIOCATALYST

以竹笋壳为原料,采用白腐菌(TST-01)对其进行预处理后,选择纤维素酶和木聚糖酶进行酶解,并在酶解产物中添加固定化混合菌种(嗜单宁管囊酵母和酿酒酵母)进行乙醇发酵。结果表明:白腐菌表现出优良的木质素降解性能,经过24d,木质素的降解率达到62.1%;6%木聚糖酶和10%纤维素酶混合,在初始pH值为4.5的条件下酶解6h,总糖转化率可达到67.54%;固定化混合菌种在发酵48h的酒精质量浓度可达到19.84g/L,高于游离混合菌种,也远高于单一菌种。

Mter being pretreated on bamboo shoots shell fiber by microbial retting, foreign xylanase and cellulose were chosen for the use of enzymatic degrading. And Pachysolen tannophilus and Saccharomyces cerevisiae yeast cells were co- immobilized by entrapment in marine alga calcium gel. Fermentation of bamboo shoots shell fiber hydrolysate was studied using the immobilized yeast cells. The experiment results indicated that the degradation rates of lignin is 62.1% after cultivation for 24d by white-rot fungi. The highest rate of saccharification reaches 67.24% at the beginning of pH 4.5, and the optimal enzyme concentrations are xylanase of 6% and cellulose of 10%, respectively. The fermentation results are superior to those obtained with suspended multi-microorganisms, or separate samples of immobilized P. tannophilus or immobilized S. cerevisiae, and the ethanol yield coefficient reaches a maximum of 19.84g/L.