摘要
建立了间接竞争化学发光酶免疫法检测赭曲霉毒素A(ochratoxin A,OTA),该方法IC50为112 pg/mL,检出限是2.47 pg/mL,平均批内和批间变异系数分别为7.03%和14.7%。在大米和小麦样本中添加浓度1.5～6μg/kg的OTA标品,平均回收率在66.97%～97.96%之间,与其他常见真菌毒素未见交叉反应。将该方法应用于30份谷物样本(包括20份大米样本和10份小麦样本)中OTA的检测,检测结果与商品化ELISA试剂盒的相关系数R2=0.942 4。该方法简单、灵敏、快速、准确适用于谷物中OTA的检测。
A sensitive indirect competitive chemiluminescence enzyme immunoassay （CLEIA）for OTA was developed. The concentration of OTA causing 50% inhibition of binding enzyme marker （ICs0） was 112 pg/mL and the detection limit was 2.41 pg/mL, the mean coefficients of variation of within groups and between groups were 7.03, 14.7% respectively. The recoveries from rice and wheat samples spiked with 1.5 ～6 μg/kg of OTA varied between 66.97% and 97.96% ,and the OTA antibody showed no cross -reactivity to the else common mycotoxins. OTA in 30 cereal samples were screened by the CLEIA, including 20 rice samples and 10 wheat samples. The results show good coefficient with commercial ELISA kit （R^2=0. 9424）. The assay was sensitive, fast and accurate, which proved to be suitable for the screening of cereal samples for the presence of OTA.
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2011年第4期204-208,共5页
Food and Fermentation Industries
基金
国家863计划项目基金(No.2007AA10Z427)
