快速液相色谱-串联质谱法测定白砂糖中的丙烯酰胺

Determination of Acrylamide in Sugar by RRLC-MS/MS

建立了白砂糖中丙烯酰胺的快速液相色谱-串联质谱(RRLC-MS/MS)测定方法。样品用乙酸乙酯提取,浓缩后用RRLC-MS/MS分析,采用电喷雾离子化(ESI+)及多反应监测模式(MRM)进行测定,用13C3-丙烯酰胺内标法进行定量。测定结果显示:在10～200 ng/mL内,标准曲线呈良好的线性关系(R2=1),样品加标平均回收率为100.5%～108.1%,定量检测限为10μg/kg,方法精密度RSD≤5.67%。

A method for the determination of acrylamide in sugar by RRLC-/MS/MS was established. After sample extracted with ethyl acetate and concentrated, it was analyzed by RRLC-MS/MS. Determinations were performed by using electrospray ionixation（ ESI^＋） and multi-reaction monitoring（MRM） modes. Internal standard of ^13C3-acrylamide was used in the quantification. The linear range of the method was 10 ～ 200 ng/mL, the recoveries were 100. 5% ～ 108.1% , the detection limit was 10 Ixg/kg and the relative standard deviations（RSD）was ≤5.67%.