噬菌体生物扩增法检测尿液结核分枝杆菌对肾结核的诊断价值探讨

The diagnostic value for renal tuberculosis by detection of Mycobacterium tuberculosis from Urine with phage amplified biologically assay

目的利用噬菌体生物扩增法检测尿液结核分枝杆菌,评价其对肾结核诊断价值。方法对肾结核患者63例应用涂片抗酸染色法检测结核分枝杆菌、结核分枝杆菌培养、噬菌体生物扩增法进行尿液结核分枝杆菌检测,聚合酶链反应法检测结核菌DNA。同时选正常人群21人作为对照组行尿液噬菌体生物扩增法检测结核分枝杆菌,聚合酶链反应法检测结核分枝杆菌DNA。结果肾结核患者尿液行噬菌体生物扩增法检测结核分枝杆菌阳性率明显高于涂片抗酸染色法检测结核分枝杆菌及结核分枝杆菌培养(P<0.01)。与PCR法比较差异无统计学意义(P>0.05),噬菌体生物扩增法检测尿液中结核分枝杆菌对肾结核诊断有较高的特异度(95.2%)和灵敏度(69.8%)。结论噬菌体生物扩增方法检测尿液结核分枝杆菌对肾结核诊断具有重要的参考价值。

Objective To evaluate the diagnostic value for renal tuberculosis by detection of Mycobacterium tuberculosis from Urine with phage amplified biologically（phaB） assay. Methods 63 patients with renal tuberculosis and 21 healthy subjects were recruited as research group and control,respectively.We detected Mycobacterium tuberculosis in the urine from research group patient using smear,culture and phaB.Meanwhile TB-DNA were detected from the urine by polymerase chain reaction（PCR）.The urine from the control were detected using phaB and PCR. Results The positive rate of detection for Mycobacterium tuberculosis from urine by phaB in research group was significant higher than that by smear and culture（P〈0.01）.But the positive rate was no significant difference between using phaB and using PCR（P〉0.05）,Specificity and sensitivity of phaB for detecting Mycobacterium tuberculosis from urine was 95.2% and 69.8%,respectively. Conclusion PhaB is useful tool to detect Mycobacterium tuberculosis from urine for diagnosis of renal tuberculosis.