妊娠高血压综合征患者胎盘滋养层细胞中血红素氧合酶-1的表达

Expression of heme oxygenase-1 protein in placental trophoblast cells of patients with pregnancy-induce hypertension

目的 观察正常晚孕妇女（NTP）及妊娠高血压综合征（PIH）患者胎盘滋养层细胞中血红素氧合酶-1（HO-1）的表达.方法 取15例NTP和PIH患者剖宫产术的胎盘绒毛小叶进行培养,应用免疫印迹法（Western Blot）检测胎盘组织中HO-1的蛋白含量.取正常早孕妇女胎盘绒毛小叶进行细胞培养,然后分别加入NTP血清（NTP组）及中重度PIH患者血清（PIH组）,进行分组培养.应用Western Blot检测HO-1的蛋白含量.结果 PIH组胎盘组织中HO-1的蛋白含量与NTP组比较差异有统计学意义（P＜0.01）;PIH患者血清培养的滋养细胞中HO-1的蛋白含量低于正常组.结论 PIH患者胎盘滋养细胞中HO-1的蛋白表达明显减少.

Objective To investigate the expression of hemo oxygenase-1（HO-1） protein in placental trophoblast cells of patients with pregnant-induced hypertension （PIH）. Methods Placental villi folial was taken from the normal terminal pregnant women and patients women with PIH for cell culture, and HO-1 protein in placental tissue was detected by Western-blot method. Placental villi folial taken from normal early pregnant women was cultured with serum of normal terminal pregnant women （NTP） and midrange and advanced PIH patients, respectively. The quantity of HO-1 protein in different group was detected with West- ern-blot. Results The expression of HO-1 protein in placental tissue of PIH patients was lower than that of NTP group （P〈0.001）. The HO-1 protein expression in placental trophoblast cells cultured with serum from PIH patients was also lower than that cul- tured with the serum of NTP woman. Conclusion The HO-1 protein expression decreases in placental villi tissue of PIH patients and the placental trophoblast cells induced by serum of PIH patient.