高效液相色谱法测定果汁和果蔬中柑橘红2号染料

HPLC Determination of Citrus Red 2 in Fruit Juices,Fruits and Vegetables

应用高效液相色谱法测定果汁、果蔬中柑橘红2号染料的含量。样品用丙酮-正己烷（1＋3）混合溶剂提取,提取液于45℃蒸发至干。用甲酸-乙腈（0.5＋99.5）溶液（经正己烷饱和）溶解残渣,正己烷脱脂。以Agilent TC C18色谱柱为分离柱,以乙腈与甲酸（0.1＋99.9）溶液按体积比92比8混合作为流动相,用二极管阵列检测器在波长508 nm处进行测定。柑橘红2号染料的质量浓度在0.25~10.0 mg·L-1范围内与其峰面积呈线性关系,测定下限（10S/N）为0.1 mg·kg-1。以果汁、鲜橙和胡萝卜样品为基体,加入3种不同浓度水平的柑橘红2号标准溶液做回收试验,测得回收率在96.3%~104.0%之间,相对标准偏差（n=6）在1.0%~3.6%之间。

HPLC was applied to the determination of citrus red 2 in fruit juices,fruits and vegetables.The sample was extracted with acetone-hexane（1＋3） solution,and the extract was evaporated to dryness at 45 ℃.The residue obtained was dissolved in formic acid-acetonitrile（0.5＋99.5） solution（saturated with n-hexane）,and defatted with n-hexane.The Agilent TC C18 column was used as stationary phase,a mixture of acetonitrile and formic acid solution（0.1＋99.9） mixed in the ratio of 92 to 8 by volume was used as mobile phase,and DAD at 508 nm was used in the determination.Linear relationship between values of peak area and mass concentration of citrus red 2 was obtained in the range of 0.25-10.0 mg·L-1,with lower limit of determination（10S/N） of 0.1 mg·kg-1.Test for recovery was made by addition of standard citrus red 2 at 3 different concentration levels to samples of fruit juice,orange and carrot as matrixes,values of recovery found were in the range from 96.3% to 104.0%.Values of RSD′s（n=6） found were in the range of 1.0%-3.6%.