顶空固相微萃取-气相色谱-质谱法测定食用植物油中挥发性有机物

GC-MS Determination of Volatile Organic Compounds in Edible Vegetable Oil with HS-SPME

提出了顶空固相微萃取-气相色谱-质谱法测定食用植物油中35种挥发性有机物（VOC′s）含量的方法。为使固相微萃取达到更高的效率,选用75μm碳分子筛-聚二甲基硅氧烷纤维作为微萃取的涂层,萃取温度及时间为90℃和30 min。用DB-5MS毛细管色谱柱分离,电子轰击离子源全扫描监测模式检测。35种VOC′s在一定的质量浓度范围内与其峰面积呈线性关系,方法的检出限（3S/N）在0.03~6.84μg·L-1之间。在3个添加水平上做回收试验,加标回收率在91.0%~108.3%之间,相对标准偏差（n=5）小于10%。

GC-MS with headspace-solid phase micro-extraction（HS-SPME） was applied to the determination of 35 volatile organic compounds（VOC′s） in edible vegetable oil.To attain a higher efficiency in SPME,the 75 μm carboxen/polydimethylsiloxane（CAR/PDMS）fiber as coating was selected for the micro-extractor,and the extraction was carried out at 90 ℃ for 30 min.The analyte were separated on DB-5MS capillary column and detected by EI ion source with whole scanning monitoring mode.Linear relationships between values of peak area and mass concentration of 35 VOC′s were kept in definite ranges.Detection limit（3S/N） found were ranged from 0.04 to 6.84 μg·L-1.Tests for recovery were performed by addition of mixed standard of 35 VOC′s at 3 concentration levels for 5 parallel determinations,results of average recovery found were in the range of 91.0%-108.3% with RSD′s（n=5） less than 10%.