健脾益气方对慢性乙型肝炎病毒携带者T细胞免疫功能的影响

Impacts of the Decoction of Fortifying the Spleen and Supplementing Qi on the cellular immunity of chronic Hepatitis B virus carriers

目的 观察健脾益气方对慢性乙型肝炎病毒（HBV）携带者T细胞免疫功能的影响.方法 将60例HBV DNA阳性慢性HBV携带者（ASC）随机分成健脾益气方治疗组（A组）、常规治疗组（B组）各30例,用流式细胞仪检测外周血T淋巴细胞亚群水平,并与健康体检者（健康对照组,n=18）进行比较;ASC治疗24周后再次检测外周血T淋巴细胞亚群.结果 慢性乙型肝炎病毒携带者外周血T淋巴细胞CD4＋、CD8＋计数与对照组差异有统计学意义（P＜0.01）;健脾益气方治疗组治疗前后T淋巴细胞CD4＋、CD8＋计数及CD4＋/CD8＋比值差异有统计学意义（P＜0.05或P＜0.01）,与常规治疗组治疗24周后T淋巴细胞计数差异有统计学意义P＜0.05）.结论 慢性HBV携带者体内存在T淋巴细胞亚群失衡,健脾益气方能够改善慢性乙型肝炎病毒携带者的T细胞免疫功能.

Objective To observe the impacts of the method of fortifying the spleen and supplementing Qi on the cellular immunity of chronic hepatitis B virus（HBV） carriers. Methods Asymptomatic HBV carriers group（ ASC group ,n = 60） with HBV DNA positive were randomly divided into Fortifying the Spleen and Supplementing Qi decoction group（ A group, n = 30） and conventional treatment group （B group, n = 30）, the peripheral blood T lymphocyte subsets were detected with flow cytometey and compared with those of the healthy people（ The normal control group, n = 18）. All the patients were treated, and 24 weeks after treatment the indexes were measured again. Results As compared with those of normal controls, the numbers of CD4＋ T cells and CD8＋ T cells were decreased significantly in HBV carriers （P 〈 0.01 ） ;There was a statistical significance between the values before and complete the treatment with the decoction of fortifying the spleen and supplementing Qi（ P 〈 0.05 or P 〈0.01 ） ,and compared with conventional treatment group,there was a statistical significance （P 〈 0. 05 ）. Conclusion T-cell subset function was disturbed in the HBV carriers. The method of foaifying the spleen and supplementing Qi could improve the cellular immunity of carriers with chronic hepatitis B.