表高油菜素内酯在满天星组培中的应用

Application of Epihomobrassiolid in the Tissue Cullture of Gysophila paniculata

表高油菜素内酯（Ｅｐｉｈｏｍｏｂｒａｓｓｉｏｌｉｄｅ，简称云大－１２０ ，或ＢＲ－ １２０） 能增加满天星组培苗的节数，并缩短节间距，使苗健壮，最适浓度为１．０ ｍｇ／Ｌ，ＢＲ－ １２０ 对组培苗的生根也有显著促进作用，最适培养基为ＭＳ＋ＢＲ－ １２０ １ ｍｇ／Ｌ，在相同浓度（０．２ ｍｇ／Ｌ）时，促进生根的效果是ＮＡＡ＞ ＢＲ－ １２０ ＞ ＩＡＡ．ＢＲ－ １２０ 对满天星组培苗的增殖无作用．在促进生根方面，ＢＲ－ １２０ 与ＮＡＡ，ＢＲ－ １２０ 与ＩＡＡ 均无明显的加成作用．

BR-120 can increase node number of Gysophila paniculata seeding which are obtained by tissue culture and can short inter node distance.Its optimum concentration is 1?mg/L,BR-120 can obviously promote rooting for the shoot,Optimum medium is MS+BR-120 1?mg/L.When the concentration of NAA,BR-120 and IAA are as same (0.2?mg/L),their rooting effect are respectively NAA>BR-120>IAA.BR-120 not has effect on promoting multiplication of the shoot.On promoting rooting of the shoot by tissue culture,there is not a obvious addition effect between BR-120 and NAA,BR-120 and IAA.