实验性变态反应性脑脊髓炎大鼠L-选择素的表达及意义

Expression of L - selectin in experimental allergic encephalomyelitis of Wistar rats and its clinical significance

目的 探讨实验性变态反应性脑脊髓炎（EAE）大鼠脑和脊髓L-选择素的表达和意义.方法 大鼠随机分为正常组、福氏完全佐剂对照组（CFA组）、左旋咪唑对照组（LMS组）和模型组 模型组采用豚鼠髓鞘蛋白匀浆和福氏完全佐剂诱发大鼠急性EAE,观察临床表现并评分 HE染色和Loyez氏髓鞘染色观察病理和髓鞘改变 免疫组化法检测脑和脊髓L-选择素表达阳性的血管数.结果 正常组和对照组大鼠均未出现症状,模型组临床症状发生率为100%,模型组L-selectin表达阳性的血管数的数值为（31.86±1.39）,较正常组（1.38±0.18）、CFA组（1.50±0.27）和LMS组（7.25±0.59）表达均明显增高（均P〈0.05）.HE染色见大脑白质及脊髓血管周围有大量炎性细胞浸润 Loyez氏染色见部分脱髓鞘改变.结论 脑和脊髓L-选择索的表达水平增高在大鼠急性EAE中发挥重要作用.

Objective To investigate the effect of L-selectin in experimental allergic encephalomyelitis （EAE） of Wistar rats.Methods The rats were randomly divided into four groups the normal group,the CFA group, the LMS group and the model group The animal models were established in rats by immunization with myelin basic protein of spinal cord of guinea pig and complete Freund＇s adjuvant（CFA）.The symptom of EAE was observed pathological feature and myelin of brain and spinal were detected with HE stain and Loyez＇s stain respectively.The number of positive vessels of L-selectin expression was detected by immunohistochemistry.Results 100% experimental Wistar rats treated with MBP and levamisole developed EAE,but none of the other groups.The number of positive vessels of L-selectin expression was （31.86 ± 1.39） in model group, obviously higher than that of in the normal group （1.38 ±0.18） ,the CFA group（ 1.50 ±0.27） and the LMS group（7.25 ±0.59） （all P 〈0.05） The inflammation cells were found around vessels and demyelination were seen in white matter of brain and spinal cords.Conclusion The expression of L-selectin should play an important role in EAE.