Galectin-9及其受体Tim-3在哮喘小鼠肺组织中的表达

Expression of Galectin-9 and Tim-3 in lungs of mice with asthma

目的探讨半乳糖凝集素-9(Galectin-9)及其受体T细胞免疫球蛋白粘蛋白分子-3(Tim-3)在哮喘小鼠肺组织中的表达及PPAR-γ激动剂罗格列酮(ROS)对其表达的影响。方法 45只清洁级雌性BALB/c小鼠随机分为对照组、哮喘组、ROS干预组。观察卵清蛋白激发及ROS干预治疗后哮喘小鼠肺组织的病理变化;应用RT-PCR方法检测肺组织Galectin-9和Tim-3 mRNA的表达;采用酶联免疫吸附测定法检测外周血IL-4及IFN-γ的表达水平。结果哮喘组肺组织Galectin-9和Tim-3 mRNA表达显著高于对照组及ROS干预组(P<0.05)。哮喘组外周血IL-4的表达量较对照组及ROS组明显升高,而IFN-γ表达量较对照组及ROS组明显降低(P<0.05)。Galectin-9和Tim-3 mRNA表达量与IL-4呈正相关(r=0.792,0.794,P<0.05),与IFN-γ呈负相关(r=-0.692,-0.757,P<0.05)。结论哮喘小鼠气道肺组织中Galectin-9和Tim-3 mRNA表达有明显升高,并且其改变与Th1/Th2细胞因子表达量具有相关性,ROS干预治疗后Galectin-9和Tim-3 mRNA表达下调。

Objective To study the expression of Galectin-9 and Tim-3 in lungs of mice with asthma and the effect of rosiglitazone（PPAR-γ agonist） on their expression.Methods Forty-five BALB/c SPF female mice were randomized into control group and asthma groups with and without rosiglitazone intervention.After ovalbumin stimulation and rosiglitazone intervention the pathological changes of the lung tissues were observed.Galectin-9 and Tim-3 mRNA levels in lung tissues were determined using RT-PCR.The levels of IL-4 and IFN-γ in peripheral blood were measured using ELISA.ResultsThe expression of Galectin-9 and Tim-3 mRNA of lung tissues in the untreated asthma group increased significantly compared with the control and the rosiglitazone treated groups（P〈0.05）.A significantly increased blood expression of IL-4 and a significantly decreased blood expression of IFN-γ were found in the untreated asthma group compared with the control and the rosiglitazone-treated groups（P〈0.05）.The expression of Galectin-9 and Tim-3 mRNA was positively correlated with blood IL-4 level（r=0.792,r=0.794 respectively;P〈0.05）,but negatively correlated with blood IFN-γ level（r=-0.692,r=-0.757 respectively;P〈0.05）.Conclusions Galectin-9 and Tim-3 mRNA levels in lungs increase in mice with asthma and significantly correlate with the levels of blood Th1/Th2 cytokines.This suggests that Galectin-9 and Tim-3 are closely related to inflammatory process in asthma.Rosiglitazone treatment may decrease the expression of Galectin-9 and Tim-3.