摘要
目的探讨中国汉族人Fas配体(FasL)基因单核苷酸多态性与重型乙型肝炎之间的关系。方法采用病例一对照研究方法,将HBV感染者作为研究对象。收集相关的非活动性HBsAg携带者233例,重型肝炎患者68例,HBV自发清除者100例,肝硬化患者102例,肝细胞癌患者112例的全血标本及临床相关资料。应用TaqMan探针实时荧光分型PCR方法检测患者Fasl基因-844T/C位点的基因多态性,计算比值比(OR)值及95%可信区间(C1)。结果应用二元Logistic回归分析,控制年龄、性别等因素后,非活动性HBsAg携带者的FasL-844CC、CT、TT基因型频率分别为50.64%、39.91%及9.44%;重型肝炎组中FasL-844CC、CT、TT基因型频率分别为79.41%、17.65%及2.94%。携带FasL-844CC基因型的非活动性HBsAg携带者与重型肝炎的发生呈显著关联(OR=4.729,95%CI:0.510~21.282,P=0.043),而其他病例对照组中存在的关联差异均无统计学意义(P〉0.05)。结论携带FasI。844CC基因型的非活动性HBsAg携带者易发生乙型肝炎重症化,应对这部分人群给予更多的关注。
Objective To investigate the relationship between single nucleotide polymorphism (SNP) of Fas ligand (FasL) and fulminant hepatitis B in Han Chinese. Methods HBV infected subjects were enrolled in this case-control study, including 233 cases of inactive HBsAg carrier, 68 patients with fulminant hepatitis B, 100 cases of spontaneous hepatitis B clearance, 102 patients with hepatitis B virus (HBV) related cirrhosis and 112 patients with HBV related primary hepatocellular carcinoma. The blood samples and clinical data were collected. FasL-844T/C polymorphisms of enrolled subjects were examined by TaqMan real time fluorescent genotyping polymerase chain reaction (RT-PCR). A adjusted odds ratios (OR) and 95 G confidence intervals (CI)were calculated using the Logistic regression model. Results After adjusting the factors of gender and age, binary Logistic regression analyses indicated that the genotype frequencies of FasL-844 CC,CT,TT in inactive HBsAg carriers were 50.64% ,39. 91% and 9. 44%, respectively, and those in cases of fulminant hepatitis B were 79. 41%, 17. 65% and 2. 94%, respectively. The analysis also revealed that FasL-844CC genotype in inactive HBsAg carriers was high risk factor of developing fulminant hepatitis B (OR = 4. 729,95%CI:0. 510-21. 282,P=0. 043), while there were no statistic significances in other cases (P〉 0. 05). Conclusion The inactive HBsAg carriers harboring FasL-844CC may have greater susceptibility to fulminant hepatitis B, which need arouse high attention.
出处
《中华传染病杂志》
CAS
CSCD
北大核心
2011年第5期286-291,共6页
Chinese Journal of Infectious Diseases
基金
基金项目:国家“九七三”高技术研究发展计划资助项目(2007CB512900)
国家自然科学基金资助项目(30872237)
关键词
肝炎
乙型
慢性
多态性
单核苷酸
配体
基因型
启动区(遗传学)
肝炎表面
抗原
乙型
Hepatitis B, chronic, Polymorphisms, single nucleotide
Ligands
Gene type
Promoter regions (genetics)
Hepatitis B surface antigens