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超级干扰素抑制A549增殖诱导其衰老 被引量：1

Super Interferon alpha Surpress the Proliferation of A549 Cells and Induce Cellular Senescence
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摘要 该研究利用MTT和结晶紫的方法证明sIFNα对肺癌细胞A549增殖有很强的抑制作用,而同样剂量的普通干扰素IFNα2b抑制作用要小很多。与对照组相比,sIFNα处理后,细胞形态发生改变,细胞体积变大,形态呈扁平状;Hoechst33258染色发现,细胞核形态无变化并且未检测到凋亡;SA-β-gal染色发现大多数细胞呈现阳性,同时,衰老相关蛋白p53和p21表达量明显上调。而IFNα2b处理组细胞形态基本没有变化,SA-β-gal染色也只有少部分的细胞呈现弱阳性。由此说明,sIFNα比IFNα2b能更好地抑制癌细胞增殖,其机制可能为诱导癌细胞发生衰老。 In this study,the anti-proliferation effect of sIFNαwas tested via the MTT assay and crystal violet staining assay.The results showed that sIFNαhad a strong anticancer effect while IFNα2b had a limited effect. After treated with sIFNα,cells possessed enlarged cell size and flatted morphologic and without nucleus morphologic alteration by Hoechst 33258 staining,and there was no detection of apoptosis.At the same time,most of sIFNα-treated cells exhibited SA-β-gal positive and with the up-expression of p53 and p21.However,cells treated with IFNα2b had little or no changes and few of them were SA-β-gal positive.In conclusion,compared with IFNα2b,sIFNαexerted a potent antineoplastic effect and the mechanism might be related to its induction of cellular senescence.
作者 李慧玲 杨冬琴 曹欣 黄宏龄 丁苗 徐海能 刘新垣 李名扬
机构地区 西南大学园艺园林学院 中国科学院上海生命科学研究院生物化学与细胞生物学研究所
出处 《中国细胞生物学学报》 CAS CSCD 2011年第5期473-478,共6页 Chinese Journal of Cell Biology
基金 国家自然科学基金(No.30623003) 国家重点基础研究发展规划(973计划)(No.2010CB529901) 国家科技重大专项(重大新药创制)(No.2009ZX09102-246) 国家高技术研究发展计划(863计划)(No.2007AA021006和2007AA02Z156) 国家科技重大专项(No.2008ZX10002-023) 中国科学院知识创新工程重要方向项目(No.KSCX2-YW-R-219) 国家自然科学基金(No.30772521)资助项~~
关键词 干扰素 衰老 抗癌 肺癌 增殖 interferon senescence anti-tumor lung carcinoma proliferation
分类号 R96 [医药卫生—药理学]
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参考文献24

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共引文献5

同被引文献21

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中国细胞生物学学报
2011年 第5期

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