2型糖尿病患者糖尿病视网膜病变不同时期外周血内皮祖细胞的数量变化

The amount of endothelial progenitor cells at different stages of diabetic retinopathy in patients with type 2 diabetes mellitus

目的 观察2型糖尿病患者糖尿病视网膜病变（DR）不同时期外周血内皮祖细胞（EPCs）的数量变化。方法 临床确诊为2型糖尿病的60例患者纳入研究。根据眼底及荧光素眼底血管造影（FFA）检查结果，将患者分为无视网膜病变（NDR）组、非增生型DR（NPDR）组及增生型DR（PDR）组，每组各20例。选择同期门诊体检正常者20名作为对照组。抽取所有受检者晨起空腹静脉血6ml，采用流式细胞仪检测，并比较4组受检者外周血EPCs数量的变化情况。结果对照组、NDR、NPDR及PDR组受检者外周血EPCs数量分别为（0．0179±0．0047）％、（0．0151±0．0086）％、（0．0123±0．1137）％、（0．0316士0．0294）％；PDR组受检者外周血EPCs数量较其他3组高，差异有统计学意义（X^2=43．780，P〈0．05）；NDR、NPDR组受检者外周血EPCs数量略低于对照组，差异无统计学意义（X^2=．244，P=0．73）；NPDR组受检者外周血EPCs数量低于NDR组，但差异也无统计学意义（X^2=6．016，P=0．12）。结论NDR、NPDR患者外周血EPCs数量降低，PDR患者外周血EPCs数量显著增高。

Objective To observe the amount of endothelial progenitor cells （EPCs） at ditterent stages of diabetic retinopathy （DR） in patients with type 2 diabetes mellitus （DM）. Methods Sixty patients with type 2 DM were divided into no DR （NDR） group, non-proliferative DR （NPDR）group and proliferative DR （PDR）group according to the examination of fundus and fundus fluorescein angiography, 20 patients in each group. Twenty healthy people were collected as the control group. 6 ml blood samples were taken from all the subjects, and then the EPCs contents in peripheral blood were detected by flow cytometry. Results The EPCs contents in peripheral blood of the control, NDR, NPDR and PDR group were （0. 0179±0. 0047） % , （0.0151±0.0086）%, （0.0123±0.1137）%, （0.0316±0.0294）%. TheEPCs contents in peripheral blood of the PDR group was significantly higher than those in others （X^2 = 43. 780, P〈0.05） ; the EPCs contents in peripheral blood of the NDR and NPDR group were slightly lower than that in the control group （X^2=5. 244, P=0.73） ; the EPCs contents in peripheral blood of the NPDR group was lower than that in the NDR group （X^2 =6. 016, P=0. 12）. Conclusion The EPCs contents in peripheral blood decreases in NDR, NPDR patients, while significantly increases in PDR patients.