落叶松八齿小蠹体内外和坑道内真菌类群

Fungal population in the inside and outside of Ips subelongatus body and the gallary of insect-bored larch logs

采集落叶松八齿小蠹成虫、幼虫、蛹,以及母坑道、子坑道、蛹室的韧皮组织和蓝变组织。采用马丁氏培养基和PDA综合培养基对各虫态体表、体内、坑道内真菌进行分离培养,统计真菌检出率。对获得的真菌菌株通过形态学和分子生物学相结合的方法进行鉴定。在落叶松八齿小蠹虫体内外和坑道内共分离出真菌31种,其中接合菌3种,子囊菌8种,担子菌1种,半知菌19种。自各虫态体表共分离出真菌23种,体内共分离出真菌8种,31种真菌在坑道内均有检出。其中,Ceratocystis fujiensis为中国新记录种,Ophiostoma sp.有待进一步鉴定,二者为在多数分离部位检出、且检出率较高的蓝变真菌,与蠹害的发生存在一定的关系。

The Ips subelongatus adult,larvae and pupa,the blue-stained tissue and phloem of pupa cells,egg and larval galleries were collected as the samples studied.The fungi in the inside and outside of the insect body and gallery of larch log were cultured and isolated by Martin and PDA medium,and the detection frequency were counted.Thirty-one species were isolated and identified,of which 3 species belong to Zygomycota,8 species Ascomycota,1 species Basidiomycota and 19 species anamorphs.All the fungi can be seen from the insect-bored gallery,23 species from the body surface of the beetle,and 8 species from the inside of the body.Ceratocystis fujiensis is a new record for China.Ophiostoma sp.is tentatively placed.Ceratocystis fujiensis and Ophiostoma sp.are blue-stained,isolated from gallery tissues,with higher frequency.