斑马鱼肠道中一株酵母菌菌株的鉴定与系统发育分析

Identification and phylogenetic analysis of a yeast strain from the intestinal tract of zebrafish Danio rereio

从斑马鱼肠道中分离到一株酵母菌,编号为ZF-5,进行了形态学观察、生理特征测定和26S rDNA D1/D2序列分析,并构建系统发育树。结果表明ZF-5菌株细胞呈卵圆形或杆状,为芽殖,有假菌丝;除乳糖外,能够发酵葡萄糖、蔗糖、麦芽糖等多种碳源;26S rDNA D1/D2区序列分析表明与季也蒙毕赤酵母Pichia guilliermondii的序列相似性最高,构建的系统发育进化树显示菌株ZF-5与Pichia guilliermondii模式菌株CBS2030(=NRRL Y-2075)亲缘关系最近,序列相似性为99.8%,因此鉴定ZF-5菌株为季也蒙毕赤酵母。

A yeast strain ZF-5 isolated from the intestinal tract of zebrafish Danio rereio was identified based on morphological and physiological characterization and 26S rDNA D1/D2 sequence analysis.The results showed that strain ZF-5 has oval to elongate cells,reproduces by multilateral budding and forms pseudohyphae.26S rDNA D1/D2 sequence of the strain ZF-5 possesses 99.8% similarity with the type strain （CBS 2030 = NRRL Y-2075） of Pichia guilliermondii.Thus this strain was identifed as Pichia guilliermondii.