ω-3多不饱和脂肪酸对胃癌细胞系生长抑制作用及其机制的研究

Apoptosis of human gastric cancer cells induced by ω-3 polyunsaturated fatty acids

目的:观察ω-3多不饱和脂肪酸(ω-3PUFAs)对MGC803低分化胃癌细胞系凋亡的影响,并对其机制进行探讨。方法:采用四甲基偶氮唑蓝(MTT)观察细胞增殖率、DNA电泳和流式细胞术(FCM)检测细胞凋亡情况、蛋白质印迹法检测凋亡蛋白PARP-1和AIF的表达。结果:二十碳五烯酸(EPA)和二十二碳六烯酸(DHA)通过诱导细胞凋亡显著抑制胃癌细胞的生长,呈时间和剂量依赖关系。DHA促进细胞凋亡的作用强于EPA。40μg/mL EPA和DHA作用MGC803细胞24 h后,凋亡相关蛋白PARP-1和AIF表达均较对照组显著增加。结论:ω-3 PUFAs通过诱导细胞凋亡抑制胃癌细胞的增殖,其机制可能通过PARP-1/AIF凋亡通路实现。

OBJECTIVE： To investigate the effect of ω-3 polyunsaturated fatty acids （ω-3 PUFAs） on the apoptosis of hu man gastric cancer cell line MGC803, and study the potential mechanisms. METHODS： Cell growth and apoptosis were analyzed with MTT assay, DNA electrophoresis and flow cytometry. Western blot was used to analyzed PARP-1 and AIF. RESULTS： Both EPA and DHA markedly inhibited the MGC803 cell growth and induced apoptosis in a time and concentration de pendent manner. The effect of DHA on apoptosis of cells was stronger than that of EPA. After incubation of the cells with 40 μg/mL EPA or DHA for 24 hours, the level of PARP-1 and AIF significantly increased. CONCLUSIONS： ω-3 PUFAs inhibit the growth of gastric cancer cells through promoting apoptosis. It in dicate that EPA/DHA appear to be mediated apoptosis of MGC803 cells line in part by the activation of PARP 1/AIF pathways.