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急性髓细胞白血病WT1基因突变的检测与临床意义 被引量：1

Detection and clinical significance of WT1 gene mutations in acute myelogenous leukemia
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摘要 WT1基因是最早发现与Wilms肿瘤发生、发展有关的基因,1990年由Call等克隆并定位于染色体11p13,全长约50 000 bp,共有10个外显子,相对分子质量为52 000～54 000,是一种锌指蛋白,也是一种双向转录调控因子,其等位基因的功能缺失在Wilms肿瘤的发生中起重要的作用[1-3].近年来发现在白血病尤其AML中WT1基因有异常的高表达,而且表达水平与预后有关,高表达可能导致较差的预后[4-5].本研究应用PCR-SSCP法对AML患者的WT1基因突变进行检测分析,以研究AML患者WT1基因突变对该疾病的发生、发展和预后评估的意义.
作者 王永伦 葛晓军 陈龙庆
机构地区 贵州省遵义医学院附属医院检验科
出处 《中华检验医学杂志》 CAS CSCD 北大核心 2011年第4期345-347,共3页 Chinese Journal of Laboratory Medicine
关键词 急性髓细胞白血病 WT1基因 基因突变 检测分析 临床意义 PCR-SSCP法 Wilms肿瘤 相对分子质量
分类号 R733.71 [医药卫生—肿瘤]
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参考文献10

  • 1王平.WT1基因与发病机制[J].前卫医药杂志,1999,10:128-129.
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  • 3Sakatani T,Shimazaki C,Hirai H,et al.Early relapse after highdose chemotherapy rescued by tumor-free autologous peripheral blood stem cells in acute lymphoblastic leukemia:importance of monitoring for WT1-mRNA quantitatively.Leuk Lymphoma,2001,42:225 -229.
  • 4秦亚溱,阮国瑞,李金兰,付家瑜,常艳,王卉,李玲娣,刘艳荣,陈珊珊.定量检测WT1基因表达水平在急性髓系白血病微量残留病监测中的意义[J].中华血液学杂志,2005,26(11):649-652. 被引量：22
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二级参考文献9

  • 1Menssen HD,Siehl JM,Thiel E. Wilms tumor gene ( WT1 ) expression as a panleukemic marker. Int J Hemato1,2002 ,76 :103-109.
  • 2Gabert J,Beillard E,van der Velden VHL,et al. Standardization and quality control studies of ‘ real-time' quantitative reverse transcriptase polymerase chain reaction of fusion gene transcripts for residual disease detection in leukemia-A Europe Against Cancer Program. Leukemia,2003,17:2318-2357.
  • 3Tamaki H,Mishima M,Kawakami M,et al. Monitoring minimal residual disease in leukemia using real-time quantitative polymerase chain reaction for Wilms tumor gene ( WT1 ). Int J Hematol, 2003, 78:349-356.
  • 4Cilloni D, Gottardi E, DeMicheli D, et al. Quantitative assessment of WT1 expression by real time quantitative PCR may be a useful tool for monitoring minimal residual disease in acute leukemia patients. Leukemia, 2002,16:2115-2121.
  • 5Ostergaa d M, Olesen LH,Kjeldsen E,et al. WT1 gene expression:an excellent tool for monitoring minimal residual disease in 70% of acute myeloid leukaemia patients-results from a single-centre study. Br J Haemato1,2004,125:590-600.
  • 6Trka J, Kalinova M, Hrusak O, et al. Real-time quantitative PCR detection of WT1 gene expression in children with AML: prognostic significance, correlation with disease status and residual disease detection by flow cytometry. Leukemia,2002,16:1381-1389.
  • 7Uzunel M, Ringden O. Poor correlation of kinetics between BCRABL and WT1 transcript levels after allogeneic stem cell transplantation. Bone Marrow Transplant, 2004, 33:47-52.
  • 8Chen Z. The possible role and application of WT1 in human leukemia. Int J Hematol, 2001,73:39-46.
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中华检验医学杂志
2011年 第4期

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