可生物降解聚乳酸载青风藤总碱微球的制备工艺

Optimization of Preparation Technology of Biodegradable PLA (Polylactic Acid) Containing Chinese Medicine Herbs

探索青风藤总生物碱微球(CSA-MS)的制备方法并优化制备工艺。采用乳化-溶剂挥发法制备CSA-MS,紫外分光光度法测定MS的包封率和载药量,扫描电镜观察MS的形貌,粒径测定仪测MS粒径分布情况,并测试药物的体外释放情况。结果显示,MS外观圆整,平均粒径为(21.5±1.22)μm。正交实验优化了MS的制备工艺,其优化条件是聚乳酸(PLA)质量分数为5%,芯/壁材比为1/10,O/W体积比为1/10。实验筛选的最佳工艺制备的CSA-MS适用于缓释药物传递系统的开发。

To explore the preparation method of microspheres containing total alkaloids of Caulis Sinomenii（CSA-MS）,optimize the preparation techniques and prescription composition.Microspheres were prepared using emulsion solvent evaporation method.The process parameters involved were investigated by UV spectrophotometric method with respect to the encapsulation and drug loading.The surface morphologies were analyzed by scanning electronic microscopy.The mean particle size was analyzed by particle size analyzer.The release experiments in vitro were carried out.The microsphere was smooth and spherical,with mean particle size of（21.5±1.22） μm.Through orthogonal test,the preparation techniques of microspheres were optimized.The optimization parameters was 5% for polylactic acid（PLA）,CSA/PLA（m/m） for 1/10,O/W（V/V） for 1/10.CSA-MS prepared by the optimizational techniques is suitable for the controlled drug release system.