转IrrE基因甘蓝型油菜对NaCl胁迫的耐受性应答

The tolerant responses to NaCl Stress in IrrE-transgenic Brassica napus

以转IrrE基因甘蓝型油菜为材料,研究了转IrrE基因甘蓝型油菜幼苗对NaCl胁迫的耐受性应答情况.在200 mmol/L NaCl胁迫下,随着胁迫时间的延长,转IrrE基因和野生型油菜的POD、SOD和CAT三种抗氧化酶活性均增加,其中野生型油菜的三种抗氧化酶在24h后达到峰值,随后逐渐下降,而转IrrE基因油菜的三种抗氧化酶活性却持续增加,并在相同的胁迫时间内均高于野生型油菜的酶活性;同时转IrrE基因油菜的脯氨酸和可溶性蛋白质含量均比野生型油菜的高,但其丙二醛(MDA)的含量比野生型油菜的低.结果表明,IrrE基因作为一种转录因子可能广泛参与了油菜幼苗对NaCl胁迫的耐受性应答过程,从而提高了植物对NaCl胁迫的耐受能力.

The tolerant responses to NaCl stress in IrrE-transgenic Brassica napus were studied. The results showed that the activity of peroxidas （POD）, superoxide dismutase （SOD） and catalase （CAT） in transgenic Brassica napus was gradually increased under 200 mmol/L NaCl during 0-48 h, but the activity of POD, SOD and CAT in non-transgenic Brassica napus was increased during 0~24 h and subse- quently decreased after 24 h. The content of proline and the dissoluble proein in transgenic Brassica hapus was higher than non-transgenic Brassica napus, but the content of malonaldehyde（MDA） in transgenic Brassica napus was lower than non-transgenie Brassica napus. Therefore, IrrE gene enhanced the activities of three antioxidant enzymes in transgenic Brassica napus, and then enhanced the tolerance of Brassica napus against NaCl stress.