黄连解毒汤对APP/PS1双转基因阿尔茨海默病小鼠脑组织自由基代谢及IL-6、IL-1β含量的影响

Effects of Huanglian-Jie-Du-Tang(HLJDT) on free radicals metabolism and IL-6 and IL-1β in PS1/APP double transgenic mice of Alzheimer's disease

目的观察黄连解毒汤(HLJDT)对阿尔茨海默病(Alzhemer’s disease,AD)模型小鼠自由基代谢及炎性细胞因子(IL-6,IL-1β)含量的影响,并探讨其可能治疗机制。方法采用APP/PS1双转基因AD小鼠模型,并随机分为模型对照组(CMC组)、阳性对照组(盐酸多奈哌齐组)、HLJDT大中小剂量组,每日予以相应药物灌胃治疗后检测脑组织中自由基代谢指标(SOD、MDA),并应用ELISA法检测炎性细胞因子(IL-6,IL-1β)含量。结果 HLJDT各剂量组均能明显提高SOD活性,降低MDA含量(P<0.01);盐酸多奈哌齐组及HLJDT各剂量组均显著降低IL-6含量(P<0.01);HLJDT小剂量组IL-1β含量低于CMC组,但HLJDT各剂量组及盐酸多奈哌齐组与模型对照组(CMC组)比较均无统计学差别(P>0.05)。结论 HLJDT治疗AD的机制可能与提高抗氧化能力,减轻炎症反应有关。

Objective To observe the effect of Huanglian-Jie-Du-Tang （ HLJDT ） on free radical metabolism and Ⅱ-6 and the levels of Ⅱ-1β in PS1/APP double transgenie mice Methods The mice were randomly divided into five groups： model control, three groups of medication by HLJDT（865 mg.kg^-1 .d^-1, 433 rrgkg^-1 .d^-1, 216 rag. kg^-1 .d^-1）, Control group （ ＋ ） with Donepezil, treated by gavage, free radical metabolism of brain were measured, ELISA was used to detect the levels of inflammatory eytokines （Ⅱ-6, Ⅱ-1β） of APP/PS1 double transgenic mice brain. Results It was showed that SOD was evidently increased and MDA was decreased in model group（ P〈O. 05 ）. The IL-6 and IL-113 levels were reduced in Donepezil group and HLJDT groups （P〈0. 01）. Conclusions The mechanism was that oxidation resistance was improved and inflammatory response was attenuated by HLJDT.