摘要
目的建立荧光素酶标记人胃癌原位异种移植模型。方法将萤火虫荧光素酶作为标记基因导入人胃癌MGC803细胞,建立稳定表达荧光素酶的细胞,将其接种裸鼠胃壁浆膜下,建立胃癌裸鼠原位肿瘤模型。用活体荧光成像系统检测肿瘤的发生发展,并进行小动物超声影像和病理学分析。结果裸鼠原位成瘤率为100%,活体荧光成像观察发现在接种第7天,就可以观察到肿瘤发光。21 d后肿瘤进入对数生长期,28 d后肿瘤出现明显坏死,平均荧光光子数呈现下降趋势。超声成像发现小鼠胃部有直径为8.39 mm,面积为28.92 mm2瘤块。结论荧光素酶标记可以实时监测原位异种移植人胃癌生长状况。
Objective To establish a human gastric cancer orthotopic xenograft model labeled with luciferase.Methods Luciferase labeled MGC803 cells were cloned with G418 selection(MGC803-Luc).MGC803-Luc cells were implanted into the stomach wall of BALB /cA-nu mice to establish the tumor-bearing mouse models.Whole-body optical imaging technique was used to monitor the growth of the implanted tumor.Results The successful rate of orthotopic transplantation was 100%.The whole-body optical imaging found that the fluorescence could be detected at 7 days after transplantation.The tumor growth became logarithmic after 21 days.However,the mean fluorescent intensity tended to decline when the tumor had apparent necrosis after 28 days.Ultrasonic imaging found a tumor on the stomach,approximately 8.39 mm in diameter and 28.92 mm2 in area.Conclusions It is possible to real-time monitoring the growth of human gastric cancer labeled with luciferase in animals.
出处
《中国比较医学杂志》
CAS
2011年第4期28-30,F0003,共4页
Chinese Journal of Comparative Medicine
基金
卫生部项目,实验动物和人类疾病动物模型资源扩展(200802036)
十一五新药专项支持(2009ZX09501-026)
关键词
人胃癌细胞
肿瘤模型
荧光素酶
生物发光
小动物超声
Human gastric carcinoma
Tumor model
Luciferase
Bioluminescent imaging
Small-animal ultrasound