荧光素酶标记人胃癌细胞裸鼠原位移植模型的建立

Establishment of a Human Gastric Cancer Orthotopic Transplantation Model Labeled with Luciferase

目的建立荧光素酶标记人胃癌原位异种移植模型。方法将萤火虫荧光素酶作为标记基因导入人胃癌MGC803细胞,建立稳定表达荧光素酶的细胞,将其接种裸鼠胃壁浆膜下,建立胃癌裸鼠原位肿瘤模型。用活体荧光成像系统检测肿瘤的发生发展,并进行小动物超声影像和病理学分析。结果裸鼠原位成瘤率为100%,活体荧光成像观察发现在接种第7天,就可以观察到肿瘤发光。21 d后肿瘤进入对数生长期,28 d后肿瘤出现明显坏死,平均荧光光子数呈现下降趋势。超声成像发现小鼠胃部有直径为8.39 mm,面积为28.92 mm2瘤块。结论荧光素酶标记可以实时监测原位异种移植人胃癌生长状况。

Objective To establish a human gastric cancer orthotopic xenograft model labeled with luciferase.Methods Luciferase labeled MGC803 cells were cloned with G418 selection（MGC803-Luc）.MGC803-Luc cells were implanted into the stomach wall of BALB /cA-nu mice to establish the tumor-bearing mouse models.Whole-body optical imaging technique was used to monitor the growth of the implanted tumor.Results The successful rate of orthotopic transplantation was 100%.The whole-body optical imaging found that the fluorescence could be detected at 7 days after transplantation.The tumor growth became logarithmic after 21 days.However,the mean fluorescent intensity tended to decline when the tumor had apparent necrosis after 28 days.Ultrasonic imaging found a tumor on the stomach,approximately 8.39 mm in diameter and 28.92 mm2 in area.Conclusions It is possible to real-time monitoring the growth of human gastric cancer labeled with luciferase in animals.