猪链球菌BALB/c小鼠感染模型的初步建立

Experimental animal models of BALB / c mice infected with Streptococcus suis

目的观察猪链球菌(Streptococcus suis)感染小鼠后的临床、病理变化,为将小鼠作为实验室研究猪链球菌的实验动物提供依据。方法用猪链球菌活菌经腹腔注射感染小白鼠,并做阴性对照,观察其临床症状,剖解病死小鼠,制作病理切片观察病理变化。并对病死小鼠做病原分离,通过培养特性鉴定、生化试验以及PCR鉴定确定其为猪链球菌。结果小鼠确由感染猪链球菌而致死,体内各组织、器官均产生典型的败血症病变。结论小鼠对猪链球菌易感,能够作为实验室研究猪链球菌良好的实验动物。

Mice were infected with living Streptococcus suis by celiac injection and the clinical symptoms were observe.The cadavers of the infected mice were anatomized to observe the pathologic changes through pathologic section preparation.According to the results of the cultural characteristics,biochemistry properties and PCR verification,the strain was identified to be Streptococcus suis.Then the mice were died from the infection of Streptococcus suis.All organs and tissues of the mice presented typical septicemic pathologic changes,proving that mouse could be a good material for study of Streptococcus suis.