Q开关1064nm Nd:YAG激光照射对大鼠皮肤中羟脯氨酸含量、皮肤厚度和FGF-1表达的影响

Impact of Q - Switched 1064 nm ND: YAG laser irradiation on the content of hydroxyproline, thickness of skin and the expression of fibroblast growth factor in rat

目的:确定Q开关1064 nm Nd:YAG激光非磨削性嫩肤对大鼠真皮胶原增生和成纤维细胞生长因子-1(FGF-1)表达的影响。方法:用Q开关1064 nm Nd:YAG 激光照射大鼠脱毛的背部皮肤,每3天1次,连续4次。照射后第14天和30天分别取大鼠皮肤测量真皮厚度并检测羟脯氨酸含量,免疫组化法检测FGF-1的表达。结果:激光照射后第14天大鼠皮肤真皮厚度明显增加,胶原纤维束粗大、密集,皮肤中羟脯氨酸含量增加,与对照组相比有显著性差异(P<0.05)。免疫组化显示FGF-1在激光照射后第14天表达明显上调。照射第30天时,真皮厚度和羟脯氨酸含量下降,FGF-1表达下调。结论:FGF-1在激光非磨削性嫩肤治疗大鼠皮肤中表达上调,可能导致真皮内胶原增生密切相关。

Objective： To determine the impact of Q - Switched 1064 nm Nd：YAG laser irradiation on the proliferation of dermal collagen and expression of fibroblast growth factor in rat skin. Methods： The depilated dorsal skin of rat was irradiated by Q- Switched 1064 nm Nd： Y AG laser： once every three day for 4 times. The thickness of skin and content of hydroxyproline were measured, and the expression of FGF - 1 tested by immunohistochemistry on the 14th day and the 30th day after irradiation separately. Results： On the 14th day after irradiation with the laser, the thickness of skin and the content of hydroxyproline were significantly increased when compared with control sites （P 〈 0.05）. The expression of FGF- 1 was upregulated with a significant difference compared with control sites （ P 〈 0.05）. The thickness of skin and the content of hydroxyproline were decreased, and the expression of FGF - 1 was down- regulated on the day 30 after irradiation. Conclusion： Q - Switched 1064nm Nd： YAG laser can markedly up - regulated the expression of FGF- 1 in rat skin, which may be the presumable mechanisms of non - ablative rejuvenation.