A型钾通道Kv4.1在戊四唑致痫大鼠海马区的表达

Expression of A-type potassium channel Kv4.1 in the hippocampus of rats with pentylenetetrazol-induced epilepsy

目的通过检测A型钾通道Kv4.1在戊四唑(PTZ)致痫大鼠海马CA1、CA3及齿状回区的表达变化,探讨A型钾通道在癫痫发病机制中的作用。方法 SD大鼠40只,随机分为正常组、致痫后1 h、24 h、72 h组。腹腔注射PTZ制备大鼠癫痫模型,应用免疫组化及Western Blot技术检测Kv4.1在各时间段海马CA1、CA3及齿状回区的蛋白表达情况。结果致痫组大鼠海马区Kv4.1蛋白表达水平在致痫后1 h、24 h、72 h三个时间段均明显高于正常组(P<0.05);各致痫组之间Kv4.1蛋白表达水平无明显差异(P>0.05)。结论大鼠癫痫模型海马区A型钾通道Kv4.1蛋白表达增多,Kv4.1的表达上调可能在癫痫的发生中起作用。

Objective To investigate the expression of A-type potassium channel Kv4.1 in the CA1,CA3 and DG regions of the hippocampus in rats with pentylenetetrazol（PTZ）-induced epilepsy.Methods Forty SD rats were randomly divided into normal control and epilepsy groups（including 3 subgroups： 1,24 and 72 hrs after the epilepsy events）.Epilepsy was induced by injecting PTZ.Kv4.1 protein was detected in the CA1,CA3 and DG regions of the hippocampus using immunohistochemical and Western Blot analysis.Results The Kv4.1 protein expression in the hippocampus in the epilepsy groups was significantly higher than in the control group（P0.05）.There were no significant differences in the expression of Kv4.1 protein among the three epilepsy subgroups.Conclusions The expression of A-type potassium channel Kv4.1 protein in the hippocampus of epileptic rats increased,and the increased expression of Kv4.1 might play a role in the pathogenesis of epilepsy.