铜绿微囊藻藻毒素提取液对小鼠淋巴细胞DNA损伤的研究

Study on DNA damages of mouse lymphocytes induced by algal toxin extraction of Microcystis aeruginosa

采用单细胞凝胶电泳(SCGE)方法,研究了实验室培养的铜绿微囊藻藻毒素MCLR粗提液、初步提取液和纯MCLR样品对小鼠淋巴细胞的毒性效应。结果显示,正己烷萃取粗提液后,其中的色素和脂类物质被大量去除。当粗提液MCLR浓度大于66.2μg·L^(-1)时,能够诱发小鼠淋巴细胞DNA损伤,初步提纯液的MCLR浓度达到125μg·L^(-1)以上,才会诱发DNA损伤,与粗提液相比,初步提纯液的tail DNA%和OTM值有所降低,但幅度不大。纯MCLR样品诱导的DNA损伤远小于粗提液,浓度为494μg·L^(-1)时的tail DNA%和OTM值与75.9μg·L^(-1)粗提液相近,在MCLR纯化过程中大量被去除的具有紫外吸收的物质是导致损伤差异的主要来源。

We studied the toxic effect of algal toxin MCLR crude extraction, preliminary purified extraction and purified MCLR produced by laboratory-cultured Microcystis aeruginosa on mouse lymphocytes by using single cell gel electrophoresis （SCGE）. Theresults showed that large amount of pigments and lipids in the crude extraction were removed by the n-hexane extraction in the preliminary process. When MCLR concentrations in crude extraction were higher than 66.2 μg.L^-1, DNA damages could be induced; whereas when MCLR concentrations in preliminary purified extraction were higher than 125μg.L^-1, DNA damages were induced. The tail DNA% and OTM of preliminary purified extraction were slightly less than those of crude extraction. However, the damages of purified MCLR were much less than those of crude extraction. The damages for purified MCLR with the concentration of 494μg.L-1 were similar to those of crude extraction with 75.9 μg.L^-1 MCLR. Major parts of organic substances with UV adsorption, which were removed in the MCLR purification process, might bring about the significant differences between the crude extraction and purified MCLR.