迷迭香对运动大鼠基础代谢水平影响及肾脏组织脂质过氧化损伤保护实验研究

The effect of rosemary on the basic metabolism level and empirical study on the protection from the lipid peroxidation damage of the kidney in the exercise rat

选用雄性实验动物大鼠(sprague dawley,SD),建立大强度耐力训练模型,研究迷迭香对脏器指数、某些激素水平、运动大鼠肾脏组织抗氧化酶活性和丙二醛(malondialdehyde,MDA)含量的影响.结果显示:迷迭香可以提高大鼠机体的脏器指数,且对肝和睾丸指数影响更为明显,对大鼠生长发育无不良影响;服用迷迭香后可以抑制大强度耐力训练引起的大鼠机体内反三碘甲腺原氨酸(rT3)和心钠素水平的下降,提高生长激素水平;在安静和运动状态下服用迷迭香后,大鼠肾脏组织中超氧歧化酶(superoxide dismutase,SOD)、过氧化氢酶(catalase,CAT)和谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)活性均有明显升高,MDA含量明显下降,运动状态下都有显著性差异(P<0.05).运动+迷迭香组与安静+迷迭香组相比,抗氧化酶活性和MDA含量变化的趋势与运动对照组和安静对照组相比变化的趋势一致.这表现迷迭香可以在正常生理范围内提高大鼠机体脏器指数,在一定程度上改善大强度耐力训练对大鼠机体激素分泌的紊乱,增强肾脏组织中的抗氧化酶活性,减轻大强度耐力训练对大鼠肾脏组织造成的脂质过氧化损伤.

This experiment objects were SD rats（♂）,the exercise models was set up by the great intensity endurance training.The effect of rosemary on the index number of organs,the level of some hormones the,antioxidase activity and MDA contents in the kidney of the rats trained with the great intensity endurance training was studied.The results displayed that rosemary could increase the index number of the exercise rats＇ organs by the great intensity endurance training.The Liver and Testicle Index were affected more obviously.Moreover,the rats＇ growth and development was had no harmful effects on.Rosemary could inhibit the level of reverse triiodothyronine and atrial natriuretic peptide to descend,which was caused by the great intensity endurance training,raise the level of growth hormone.Rosemary could also reinforce in different level the activity of antioxidase SOD、CAT and GSH-Px and degrade the content of MDA under the resting state and kinestate,especially under the kinestate,displaying significant differences（P0.05）.Compareing the exercise＋rosemary group and the quiet＋rosemary group,the changed tendency of the antioxidase activity and MDA contents was coincident.The rosemary can increase the index number of the exercise rats＇ organs in the normal physiological limit,improve in some extent the disturbance of hormone secretion caused by the great intensity endurance training,reinforce the activity of antioxidase,and relieve the peroxidation damage to kindey resulted in by the great intensity endurance training.