中药复方“松友饮”联用干扰素α影响姑息性肝切除术后残癌生长转移的实验观察

Experimental observation of the effects of Chinese herbal compound "Songyou Yin" combined with interferon-alpha on the growth and metastasis of residual hepatocellular carcinoma in nude mice model of palliative resection

目的研究中药"松友饮"联用干扰素(IFN)α对高转移人肝细胞癌(HCC)裸鼠原位移植瘤姑息性肝切除模型残癌生长、转移和生存期的影响。方法以高转移人肝癌MHCC97H细胞建立裸鼠原位移植瘤姑息性肝切除模型,72只成模裸鼠随机分成对照组、"松友饮"组、IFNα组及"松友饮"+IFNα组(每组18只),姑息性肝切除术后24 h开始每天1次灌胃给药及皮下注射,连续5周测量裸鼠体质量。最后一次处理结束48 h后每组随机处死6只裸鼠,计算肿瘤体积及转移情况;剩余裸鼠用于观察生存期。结果对照组、"松友饮"组、IFNα组及"松友饮"+IFNα组肿瘤体积分别为(1205.2±581.3)、(724.9±337.6)、(507.6±367.0)、(245.3±181.2)mm3(F=6.410,P<0.05);肺转移结节数(n)分别为13.8±3.5、12.1±3.8、7.4±2.8、4.5±1.9(F=22.930,P<0.05);生存期分别为(62.1±2.0)、(76.2±3.2)、(81.1±2.5)、(88.3±2.5)d(Chi-Square=45.704,P<0.05)。联合用药组同单药组比较,疗效差异有统计学意义。结论连续5周联用"松友饮"增强IFNα抑制姑息性肝切除术后残癌生长、转移并延长荷瘤裸鼠生存期,对HCC患者有潜在临床应用价值。

Objective To investigate the effects of combined therapy of Chinese herbal compound ＂Songyou Yin＂ and IFNα on residual tumor growth,metastasis and survival in nude mice following palliative resection.Methods Nude mice models of human hepatocellular carcinoma（HCC） were established via orthotopic implantation of a highly metastatic potential human HCC cell line MHCC97H.Seventy-two nude mice which underwent palliative liver resection were randomized into control group,＂Songyou Yin＂ group,IFNα group and the combined group（＂Songyou Yin＂＋ IFNα）.Daily intragastric administration of ＂Songyou Yin＂ and injection of IFNα were given for 5 consecutive weeks 24 hours after palliative resection.Daily general observations and weekly body weights were recorded.Six mice in each group were sacrificed 48 h after the final treatment to examine tumor volume and lung metastasis.The remaining mice were used to observe life span.Results The HCC tumor volume was（1205.2±581.3）,（724.9±337.6）,（507.6±367.0） and（245.3±181.2） mm3 in control group,＂Songyou Yin＂ group,IFNα group and the combined group（F=6.410,P0.05） respectively,accorded with the number of pulmonary metastatic nodules（n） of 13.8±3.5,12.1±3.8,7.4±2.8 and 4.5±1.9（F=22.930,P0.05）.The life span（Means ± SE） was（62.1±2.0）,（76.2±3.2）,（81.1±2.5） and（88.3±2.5）d respectively（Chi-Square=45.704,P0.05）.Statistical significance could be seen as comparison was made between the combined group and the single drug therapy groups.Conclusion Five weeks administration of Chinese herbal compound ＂Songyou Yin＂ reinforced the inhibition effects of IFNα on the residual HCC growth and lung metastasis,and extension of life span in nude mice model of palliative resection,which indicated its potential use in patients with HCC.