高压氧对大鼠急性脊髓损伤炎症因子表达的影响

Effects of hyperbaric oxygen on the expressions of inflammatory factors in case of acute spinal cord injury in rats

目的观察大鼠脊髓损伤（spinal cord injury，SCI）后早期高压氧（HBO）治疗对肿瘤坏死因子-α（TNF-α）、白细胞介素-10（IL-10）mRNA表达的影响。方法将36只sD大鼠随机分为2组：SCI组（18只）和HBO组（18只）。2组均采用改良的Allen打击法建造大鼠脊髓损伤模型。模型建造成功后，HBO组于损伤2h后开始行HBO治疗，1次／d。2组分别于损伤后6、12、24、72、120、168h各取3只大鼠，取损伤部位脊髓组织采用逆转录一聚合酶链式反应（RT—PCR）法测定其中TNF-α、IL-10mRNA表达的变化。结果SCI组大鼠脊髓组织中TNF-α／mRNA表达逐渐升高，在伤后12h表达明显上调，至24h达高峰，高表达持续至损伤后72h；HBO组大鼠脊髓组织中TNF-α mRNA表达变化趋势与SCI组一致，但升高幅度降低（P〈0．05）；SCI组大鼠IL-10mRNA表达在损伤后12h开始升高，并随时间的推移逐渐升高，至168h达高峰；HBO组表达变化趋势较一致，IL-10mRNA升高幅度更明显（P〈0．05）。结论HBO能够减少前炎性细胞因子的释放，增加抗炎性细胞因子的表达，从而减少脊髓组织的继发损伤，保护受损的神经细胞，达到促进恢复的作用。

Objective To investigate the effects of early HBO therapy on the expressions of proinflammatory eytokine mRNA including tumor necrosis faetor--α（TNF--α） and interleukin-10 （IL-10）following spinal cord injury （SCI） in rats. Methods Forty SD rats were randomly divided into 3 groups： the sham operation group （n =4）, the SCI group （n = 18）, and the hyperbaric oxygen group （n = 18）. Spinal cord injury model was developed by using the modified Allen impact. Then, the SCI group and the HBO group received HBO therapy 2 hours after injury, once a day. And 3 rats were randomly selected at 6, 12, 24, 72, 120 and 168 h following injury to take samples of injured spinal cord tissue and measure dynamic changes in the expressions of TNF--α, IL-lO mRNA by semi-quantitative RT-PCR method. Results Faint expression of the cytokine mRNA could be noticed in the sham group. The expression of TNF-a mRNA in the injured spinal cord tissue in the SCI group elevated gradually, increased obviously at 12 h after injury and reached peak at 24 h, and its high expression maintained till 72 h after injury. The tendency in the expression of TNF-α mRNA in the HBO group was identical to that of the SCI group, however, the amplitude in the increase of TNF-α mRNA decreased（P 〈0. 05）. The expression of IL-10 mRNA in the SCI group began to increase at 12 h after injury and increased gradually over time and reached peak at 168 h. The expressions of both TNF-α and IL-10 mRNA were more consistent in the HBO group, with more obvious increase in the expressions of IL-10 mRNA. Conclusions HBO could reduce the release of pro-inflammatory cytokines and increase the expression of anti-inflammatory cytokines, resulting in reduction of secondary spinal cord injury, protection of the damaged nerve cells and promotion of recovery.