鲨鱼硫酸软骨素两种测定方法的比较研究

A comparative study on the two methods for the determination of the shark chondroitin sulfate

目的比较2种鲨鱼硫酸软骨素含量的测定方法。方法以硫酸软骨素、氨基己糖为标准品，采用咔唑法、Elson—Morgan法分别测定鲨鱼软骨提取物中硫酸软骨素的含量。结果咔唑法在4．710～47．100μg／ml范围内，线性关系良好（r=0．9999），溶液在3h内稳定性良好，相对标准差RSD=1．21％，重现性试验RSD=1．36％（n=6），精密度试验RSD=0．46％（n=6），平均回收率为99．60％，RSD=1．39％（n=6）。Elson—Morgan法在2．552～25．520μg／ml范围内，线性关系良好（r=0．9996），溶液在3h内稳定性良好，RSD=1．62％，重现性试验RSD=2．61％（n=6），精密度试验RSD=1．07％（n=6），平均回收率为96．55％，RSD=2．43％（n=6）。结论咔唑法测定鲨鱼硫酸软骨素含量较Elson—Morgan法简便、快速、准确，可作为测定鲨鱼硫酸软骨素含量的方法。

Objective To compare the two methods for the determination of the shark chondroitin sulfate. Methods Using chondroitin sulfate and aminohexose as standards to determine the content of the shark chondroitin sulfate by the carbazole method and the Elson-Morgan method. Results Experiments showed that by the carbazole method, the concentration of the shark chondroitin sulfate was within the ranges of 4. 710- 47. 100μg/ml, displaying good linear relationship （ r = 0. 9999 ） and good stability within 3 hours, with RSD = 1.21% ,reproductive test RSD = 1.36% （ n = 6 ） , precision test RSD = 0. 46% （ n = 6 ）, and a recovery rate of 99. 60% , RSD =1. 39% （n = 6）. For the Elson-Morgan method, the concentration of the shark ehondroitin sulfate was within the ranges of 2. 552-25. 520μg/ml, also displaying good linear relationship （ r = 0. 9996） and good stability within 3 hours, with RSD = 1.62% , reproductive test RSD = 2. 61% （n = 6）, precision test RSD = 1.07% （n = 6 ）, and a recovery rate of 96. 55% , RSD = 2. 43 % （n = 6 ）. Conclusions When compared with the Elson-Morgan, the earbazole method was more simple, rapid and accurate and could be used a standard method for the determination of the content of shark chondroitin sulfate.