白三烯、IL-8和TNF-α在银屑病患者血、尿和皮损中的表达

Expression of leukotnenes, IL - 8 and TNF - α in patients with psoriasis

目的:检测白三烯(LTs)、IL-8和TNF-α在银屑病中的表达。方法:采用ELISA方法分别检测32例银屑病患者血清、尿液和皮损中LTB4、LTE4水平及血清、皮损中IL-8和TNF-α水平,并与20例健康志愿者作对照。结果:患者血清、皮损中IL-8、TNF-α和血清、尿液、皮损中LTE4水平均明显高于对照组(均P<0.01),皮损中LTB4水平明显升高(P<0.01),但血清和尿液中LTB4水平与对照组相比差异无显著性(P>0.05)。皮损中LTB4、LTE4和IL-8水平与银屑病皮损面积与严重性指数(PASI)评分变化趋势一致,均有显著相关性。结论: 白三烯通过参与局部炎症反应在银屑病发病中起重要作用。

Objective： To detect the expression of leukotrienes（LTs）, IL- 8 and TNF- a in patients with psoriasis. Method： ELISA method was used to detect the levels of LTIM and LTE4 in serum, urine and skin lesion, and the levels of IL - 8, TNF - α in serum and skin lesion in 32 psoriasis patients and 20 healthy controls. Results： The levels of IL - 8 and TNF - α in serum and skin lesion, and the level of LTE4 in serum, urine and skin lesion were significantly higher in psoriasis patients than in controls （P 〈 0.01 ,respectively）. The level of LTB4 in skin lesion was significantly increased （ P 〈 0.01 ）, but levels of LTIM in serum and urine were not significantly increased （ P 〉 0.05, respectively）. There was a positive correlation between the levels of LTB4, LTE4 and IL- 8 in skin lesion and psoriasis area and severity index （PASI）. Conclusion： LTs play an important role in the pathogenesis of psoria- sis by participating local inflammatory reactiorLs.