树突状细胞体外诱导抗肿瘤免疫

Dendritic Cells in vitro Inducing Efficient and Specific Antitumor Immune Response

目的以树突状细胞（DC）在体外诱导抗肝癌免疫。方法自肝癌患者外周血中分离DC；以粒／巨噬细胞集落刺激因子（GM－CSF）及白介素-4（IL－4）联合刺激DC；以人肝癌细胞系HepG2细胞的肿瘤相关抗原（TAA）激活DC；DC诱导自体T淋巴细胞增殖、分化为细胞毒性T细胞（CTL）；检测CTL及其上清液对HepG2细胞、BEL－7402细胞、LOVO细胞及HOS－8603细胞的细胞毒作用。结果经人肝癌细胞系HePG2细胞的TAA激活并经GM－CSF及IL－4联合刺激后，肝癌患者外周血DC能够诱导自体T淋巴细胞增殖分化为CTL，该CTL及其上清液对HePG2细胞均有高效杀伤作用（杀伤率分别为92％±10％和41％±8％），对BEL－7402细胞亦有较强的杀伤力（杀伤率分别为56％±10％和31％±9％），对SGC－7901细胞、LOVO细胞及HOS－8603细胞则无明显的细胞毒作用。结论肝癌患者外周血DC体外能够诱导高效而特异抗肝癌免疫。提示DC可能在肿瘤治疗及预防其复发和转移中发挥重要作用。

Objective To induce efficient and specific antitumor immune response in vitro with dendritic cell (DC). Methods We isolated and proliferated DC from peripheral blood of hepatocellular carcinoma (HCC) patients .stimulated the DC with tumor-associated antigen(TAA) from human HCC cell line HepG2, activated the DC with combination of granulocyte/macrophage colony stimulating factor (GM-CSF) and interleukin 4(IL-4). Then DC was used to induce T lymphocyte to proliferate and differentiate cytotoxical T lymphocyte (CTL),the HepG2 cells being killed by CTL and its supernate. Results CTL and its supernate induced by the DC which stimulated by TAA from HepG2 cells could selectively kill HepG2 cells (killing rate was 92% ±10% and 41 % ±8% respectively)and also kill BEL-7402 cells (killing rate was 56% ±10% and 31 % ±9% respectively), but slightly killed SGC-7901 cells,LOVO cells and HOS-8603 cells. Conclusion DC from HCC patients can induce efficient and special immune response against HCC,which suggests that DC might play an important role in the treatment and prevention against tumor.[