阿霉素与β-胡萝卜素联合应用对人肝细胞癌治疗的实验研究

Experimental Study on the Treatment of Human Hepatocarcinoma in Nude Mouse by Beta-carotene Combined with Adriamycin

目的研究β-胡萝卜素（BC）和／或阿霉素（ADM）对裸小鼠人肝细胞癌组织模型（LTNM4）的治疗作用。方法第三次化疗后72小时处死全部裸鼠取血，采用酶联免疫吸附法（ELISA）检测血清甲胎蛋白（AFP），利用流式细胞仪（FCM）检测肿瘤及骨髓组织细胞周期分布情况。结果（1）BC单独使用对血清AFP水平无明显影响，当BC与ADM联合应用后，其血清AFP水平（2574．50ng／ml±431．53）较单独应用ADM（3727．03ng／ml±1498．67）显著降低（P＜0．05）。（2）人肝癌细胞FCM检测结果BC使G0／G1％显著升高（P＜0．01）；ADM使G2M％显著升高（P＜0．01）；BC与ADM联合应用后，S％、G2M％显著升高（P＜0．05，0．01）。（3）骨髓细胞FCM检测。BC对骨髓细胞周期分布无影响．ADM使S％、G2M％显著升高（均P＜0．01）．BC与ADM联合应用后，S％、G2M％较单用ADM显著降低（均P＜0．05）。结论（1）ADM与BC联合应用对抑制LTNM4肝癌细胞生长及其AFP分泌具有协同作用，BC可显著增强化疗疗效；（2）BC可显著缓解ADM所致裸鼠骨髓损伤．对骨髓具有保护作用。

Objective To study the effects of beta-carotene (BC) and/or adriamycin (ADM )on human hepatocarcinoma in nude mouse. Methods Serum alpha-fetoprotein (AFP) by enzyme linked immunosorbent assay(ELSIA) .and the cell cycle of the tumor tissue and femoral bone marrow were measured by flow cytometry (FCM ). Results (1)BC had no statistical effect on the serum AFP level;the BC combined with ADM decreased the serum AFP level than ADM did alone (P<0. 05),the serum AFP level being 2574. 50ng/ml ± 431. 53 and 3727. 03ng/ml ± 1498. 67 respectively. (2)The tumor tissue was examined by FCM. It was found that BC increased the percentage of G,/G, cell (P<0. 01 ),ADM increased the percentage of G2M cell (P<0. 01 ),and after BC combined with ADM,the percentage of S cell and G2M cell increased significantly (P<0. 05. 0. 01 ). (3)BC had no effect on the femoral bone marrow cell cycle,ADM increased the percentage of S cell and G2M cell decreased significantly (P<0. 05,respectively). Conclusion (1)The human hepatocarcinoma growth and AFP excretion were inhibited singniflcantly by BC combined with ADM. (2)BC could relieve ADM toxic effects on bone marrow.[