期刊文献+

磷酸酶PPM1G与IKKi相互作用下调IRF的转录活性

PPM1G Interacts with IKKi and Inhibits the Transcriptional Activity of IRF
下载PDF
导出
摘要 为了研究磷酸酶PPM1G对IRF信号通路的影响,首先在真核细胞中成功表达了HA-PPM1G蛋白质。应用双荧光报告系统,研究了PPM1G对IRF信号通路的影响。结果显示,过表达PPM1G能够显著抑制IKKi激活的IRF转录活性。进一步免疫共沉淀的实验结果表明,PPM1G和IKKi在细胞内存在相互作用。 To study the effect of phosphatase PPM1G on IRF signaling pathway,HA-PPM1G protein in eukaryocytes was overexpressed,and Dual-Luciferase Reporter Assay System was used to evaluate the effect of PPM1G on transcriptional activity of IRF.The activation of IRF signaling pathway stimulated by TRIF or IKKi overexpression is found that overexpression of PPM1G inhibited,and further immuno-precipitation experiment revealed that PPM1G specifically interacted with IKKi in vivo.
出处 《科学技术与工程》 2011年第14期3147-3150,共4页 Science Technology and Engineering
基金 国家自然科学基金(30871234)资助
关键词 PPM1G IKKi IRF 转录活性 PPM1G IKKi IRF transcriptional activity
  • 相关文献

参考文献10

  • 1Honda K, Takaoka A,Taniguchi T. Type I interferon gene induction by the interferon regulatory factor family of transcription factors. Immunity,2006 ; 25 : 349-360.
  • 2Shimada T, Kawai T, Takeda K, et al. IKK-i, a novel lipopolysac- charide-inducible kinase that is related to IκB kinases. Int Immunol, 1999 ; 11 (8) :1357-1362.
  • 3Fitzgerald K A, McWhirter S M, Faia K L, et al. IKKe and TBK1 are essential components of the IRF3 signaling pathway. Nat Immunol,2003; 4 (5): 491-496.
  • 4Travis S M, Welsh M J. PP2C gamma: a human protein phosphatase with a unique acidic domain. FEBS Lett, 1997; 412(3): 415-9.
  • 5Murray M V, Kobayashi R, Krainer A R. The type 2C Ser/Thr phosphatase PP2Cgamma is a pre-mRNA splicing factor. Genes Dev, 1999 ; 13 : 87-97.
  • 6Allemand E, Hastings M L, Murray M V, et al. Altemative splicing regulation by interaction of phosphatase PP2Cgamma with nucleic acid-binding protein YB-1. Nat Struct Mol Biol, 2007; 14:630-638.
  • 7Kimura H, Takizawa N, AUemand E, et al. A novel histone exchange factor, protein phosphatase 2Cγ, mediates the exchange and dephosphorylation of H2A-H2B. J Cell Biol, 2006; 175:389-400.
  • 8Guthridge M A, Bellosta P, Tavoloni N, et al. FIN13, a novel growth factor-inducible serine-threonine phosphatase which can inhibit cell cycle progression. Mol Cell Biol, 1997; 17:5485-5498.
  • 9Suh E J, Kim T Y, Kim S H. PP2Cgamma-mediated S-phase accumulation induced by the proteasome-dependent degradation of p21 ( WAF1/CIP1 ). FEBS Lett,2006 ; 580 : 6100-6104.
  • 10Petri S, Grimmler M, Over S, et al. Dephosphorylation of survival motor neurons (SWN) by PPM1G/PP2Cγ governs Cajal body localization and stability of the SWN complex. Journal of Cell Biology, 2007; 179 : 451-465.

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部