肝细胞癌Autotaxin cDNA部分序列测定及其意义

A study on expression of autotaxin mRNA in hepatocellular carcinoma

目的进行肝癌Ａｕｔｏｔａｘｉｎ（ＡＴＸ）ｃＤＮＡ部分序列测定，观察ＡＴＸｍＲＮＡ在肝癌组织中的表达程度，以探讨ＡＴＸ在肝癌转移机理中的作用。方法提取肝癌７７２１细胞株、５例正常肝组织、３２例肝癌组织ＲＮＡ，参照黑色素瘤ＡＴＸｃＤＮＡ序列设计引物，经逆转录聚台酶链反应（ＲＴ－ＰＣＲ）扩增肝癌ＡＴＸ，以卜ａｃｔｉｎ基因为内参照进行ＡＴＸｍＲＮＡ表达程度分析，同时７７２１细胞株ＲＴ－ＰＣＲ产物进行ｃＤＮＡ序列测定。结果肝癌７７２１细胞株３２例肝癌组织５例正常肝组织中均有ＡｕｔｏｔａｘｉｎｍＲＮＡ表达，其ｃＤＮＡ序列测定（３３ｈｐ）显示与黑色素瘤具有９９．７％的同源性；癌组织ＡＴＸｍＲＮＡ表达显著高于正常肝组织（分别为０．６８±０．１和０．３１±０．０８）。结论肝癌及正常肝组织表达ＡＴＸｍＲＮＡ，ＡＴＸ可能参与了肝癌的浸润与转移过程，在其中扮演了重要角色。

Objective To know if ATX is expressed in hepatocellular carcinoma. Methods 7721hepatoma cell lines in culture, 5 normal liver tissues, and 32 histologically verified HCC specimens wereobtained. Semiquantitative reverse transcription polymerase chain reaction was used to detect mRNA expression of ATX. Results The cDNA sequence of RT--PCR product of 7721 hepatoma cell lines wasmeasured, it showed that nucleotides measured were 332hp. ATX cDNA nucleotide wnuence in HCC wasfound to share 99.7% homology with that in Melanoma. ATX was expressed in all of the 32 HCC and 5normal liver tissues. The mean expression level of ATX bine in HCC samPles was higher than that innormal liver tissues (0.68 ± 0.1 vs 0.3 ± 0.08, p<0.01). Conclusion ATX bine was found to be in existencein HCC and normal liver tissues. The homology of ATX cDNA sequence between HCC and humanMelanoma was 99.7%, and ATX may play an important role in HCC metastatic mechanism.