人乳头状瘤病毒感染对孕妇和绒毛滋养细胞的影响

Effects of Human Papillomavirus Infection on Pregnant Women and Chorionic Trophoblasts

目的 探讨人乳头状瘤病毒（ Ｈ Ｐ Ｖ） 感染对孕妇和绒毛滋养细胞的影响。方法 观察６２例早、中期孕妇尖锐湿疣的病理形态特点，用 Ｈ Ｐ Ｖ６／１１ Ｄ Ｎ Ａ 探针原位杂交法检测其绒毛、胎盘组织及其中１０ 例外阴、阴道、宫颈尖锐湿疣组织，并用 Ｈ Ｐ Ｖ６／１１ 聚合酶链反应（ Ｐ Ｃ Ｒ） 方法验证１０ 例绒毛及胎盘组织。结果 孕期尖锐湿疣病变发展快，多部位发生比例高，大体形态、组织形态典型者多。 Ｈ Ｐ Ｖ６／１１ Ｄ Ｎ Ａ 原位杂交和 Ｐ Ｃ Ｒ 检测绒毛及胎盘组织 Ｈ Ｐ Ｖ 均呈阴性，尖锐湿疣组织中 Ｈ Ｐ Ｖ 阳性８ 例。结论 早、中期妊娠时， Ｈ Ｐ Ｖ 感染不致造成宫内垂直传播，对绒毛滋养细胞及胎儿不构成潜伏感染的危险。

Objective To evaluate the effects of human papilloma virus (HPV) infection on pregnant women and chorionic trophoblasts. Methods The morphological characters were observed from 62 pregnant women with condyloma acuminatum during the first and second trimester. In situ hybridization (ISH) was performed to detect HPV 6/11 in chorionic villi of all these cases. In 10 cases, ISH was also performed in condyloma acuminatum of vulva, vaginal and cervix. In 10 cases HPV 6/11 was detected by polymerase chain reaction (PCR) in chorionic villi as well. Results The lesions condyloma acuminatum developed rapidly during pregnancy. Multiple lesions were found usually, and the gross appearances and histological features were more typical. Using ISH and PCR to detect HPV 6/11 DNA in chorionic villi all showed negative. 8 of 10 cases were positive in condyloma acuminatum sites by ISH. Conclusions HPV infection does not make vertical transmission during first and second trimester. It does not increase the potential risk for the fetus.