脂质体-C-erbB2反义脱氧寡核苷酸对卵巢癌细胞的作用

Effect of Liposome C erbB2 Antisense Oligodeoxynucleotides on Human Ovarian Cancer Cells

目的 探讨脂质体 Ｃｅｒｂ Ｂ２ 反义脱氧寡核苷酸（ 脂质体 Ｃｅｒｂ Ｂ２ Ｓ Ｏ Ｄ Ｎｓ） 对卵巢癌细胞系 Ｃｅｒｂ Ｂ２ 癌基因表达及细胞增殖的影响。方法 应用流式细胞仪技术，３ Ｈ胸腺嘧啶核苷掺入方法，观察脂质体 Ｃｅｒｂ Ｂ２ Ｓ Ｏ Ｄ Ｎｓ 对卵巢癌细胞系 Ｓ Ｋ Ｏ Ｖ３ Ｃｅｒｂ Ｂ２ 癌基因表达及细胞生长、细胞周期的作用。结果 脂质体 Ｃｅｒｂ Ｂ２ Ｓ Ｏ Ｄ Ｎｓ 能够降低 Ｃｅｒｂ Ｂ２ 的表达，并抑制细胞生长；脂质体 Ｃｅｒｂ Ｂ２ Ｓ Ｏ Ｄ Ｎｓ降低细胞 Ｃｅｒｂ Ｂ２ 癌基因表达的作用效率较单用 Ｃｅｒｂ Ｂ２ 反义脱氧寡核苷酸（ Ｃｅｒｂ Ｂ２ Ｓ Ｏ Ｄ Ｎｓ）增强约４０ 倍。结论 Ｃｅｒｂ Ｂ２ 癌基因的反义调控在卵巢癌基因治疗中有一定作用；脂质体可有效增强 Ｃｅｒｂ Ｂ２ Ｓ Ｏ Ｄ Ｎｓ 调控细胞 Ｃｅｒｂ Ｂ２ 癌基因表达的作用。

Objective To explore the effects of liposome C erbB2 antisense phosphorothioate oligo deoxynucleotides(S ODNs)on C erbB2 protooncogene expression and cell proliferation in human ovarian cancer cells. Methods The effects on C erbB2 protooncogene expression, cell proliferation and cell cycle in human ovarian cancer cells were studied by flow cytometry and 3H thymidine incorporation. Results Liposome C erbB2 S ODNs could reduce C erbB2 expression and inhibit cell proliferation in human ovarian cancer cells; the effectiveness of liposome C erbB2 S ODNs on the expression of C erbB2 was much higher than that of C erbB2 S ODNs, about 40 times. Conclusions The data in this study suggest that antisense therapy is an useful method of gene therapy in ovarian cancer. The effectiveness of C erbB2 S ODNs could be greatly increased by C erbB2 S ODNs encapsulated in liposomes.