退变颈椎间盘致炎机制的实验研究

Experimental study on the inflammatory mechanism of degenerated cervical intervertebral disc

目的：检测退变颈椎间盘中炎症反应标志物含量变化，从生物化学角度探讨颈椎病的发生机制。方法：２０ 只新西兰兔随机分为造模与对照组２ 组，每组再分３ 个月和８ 个月２ 个观察亚组。切除造模组动物颈棘上、棘间韧带及分离颈椎后旁两侧肌肉，造成颈椎力学上的失衡而诱导兔颈椎间盘退行性改变。在术后 ３ 个月和 ８ 个月时分别处死动物，取颈椎间盘组织，行病理学检查并测定其中组织胺、５羟色胺、 Ｐ Ｇ Ｅ２ 和６酮 Ｐ Ｇ Ｆ１α含量。结果：造模组动物颈椎间盘中４ 种炎症介质含量均明显高于相应观察点的对照组；而造模组动物术后８ 个月的颈椎间盘中 ４ 种炎症介质含量均与术后 ３ 个月无显著差异（ Ｐ＞ ０．０５）。结论：退变颈椎间盘是致炎的，退变颈椎间盘中产生的炎症介质可能直接参与颈椎病的发病过程。

Objective: To demonstrate whether some important inflammatory markers rise in degenerative cervical intervertebral disc, and to investigate pathogenesis of cervical spondylosis in biochemistry. Methods: In this experiment,20 white New Zealand rabbits were used and divided into 2 groups: control group and model group. Each group consisted of 2 subgroup: 3 month group and 8 month group. The experimental model of degenerative cervical disc in the rabbit was established by resection of the cervical supraspinous and interspinous ligaments and detachment of the posterior paravertebral muscles from the cervical vertebrae. Then animals in each group were killed 3 months and 8 months after operation, respectively. The concentrations of histamine,5 OH tryptamine,PGE 2 and 6 keto PGF 1α were assayed in all animal disc samples. Results: The contents of 4 inflammatory mediators from the discs of model groups were overtly higher than that of control groups, respectively. The contents of 4 inflammatory mediators had no statistical difference between model groups of 3 month and 8 month. Conclusion: The degenerated cervical disc is inflammatory, and the inflammatory mediators produced by the degenerated cervical intervertebral disc may be directly related to the pathogenesis of cervical spondylosis