γ射线照射后小鼠骨髓造血细胞凋亡及Fas表达的研究

Apoptosis of bone marrow hematopoietic cell and Fas expression in 60 Coγ irradiated mice

目的：研究γ射线照射后小鼠骨髓造血细胞凋亡及其与 Ｆａｓ 表达的关系。方法：采用电镜、 Ｄ Ｎ Ａ 琼脂糖凝胶电泳及流式细胞术观察小鼠经６０ Ｃｏ γ射线照射后不同时间的骨髓造血细胞凋亡及 Ｆａｓ 的表达情况。结果：在照射后４ ｈ 细胞即出现核固缩、染色质凝集； Ｄ Ｎ Ａ 琼脂糖凝胶电泳有大量的小分子片段，呈典型“梯状”图谱；流式细胞术分析表明，凋亡细胞百分率在照射后４ ｈ 明显增加，８ ｈ 达到高峰；照后４ ｈ， Ｆａｓ 表达显著增强。结论：γ射线照射后可引起骨髓造血细胞凋亡， Ｆａｓ 可能参与了辐射诱导的造血细胞凋亡过程。

Objective:To study the apoptosis of bone marrow hematopoietic cells and Fas expression in 60 Coγ irradiated mice. Methods: Mice were whole body irradiated with 4.0 Gy γ rays.The bone marrow hematopoietic cells were analyzed by electron microscopy(EM),electrophoretic analysis of DNA and flow cytometry. Results:EM showed that there were nuclear contraction and chromatin condensation.Agarose gel electrophoresis of DNA showed ladder pattern.The peak percentage of apoptotic cells occurred 8 h after irradiation. Fas expression significantly increased in bone marrow hematopoietic cells than that of control group 4 h after irradiation. Conclusion: The results indicate that apoptosis occurs in bone marrow hematopoietic cells of mouse with γ irradiation.The expression of Fas may play a role in raidation induced apoptosis of hematopoietic cells.