广西孕妇乙型肝炎病毒宫内感染及其危险因素的研究

A STUDY ON THE HEPATITIS B VIRUS INTRAUTERINE INFECTION AND ITS RISK IN GUANGXI

目的：为深入揭示乙型肝炎病毒（ＨＢＶ）宫内感染及其危险因素。方法：采用多聚酶链反应（ＰＣＲ）技术结合酶联免疫吸附法（ＥＬＩＳＡ）对广西１１６例ＨＢｓＡｇ阳性和５２例ＨＢｓＡｇ阴性孕妇外周血及其引产胎儿胎心血进行ＨＢＶ血清学标志物和ＨＢＶＤＮＡ检测。结果：ＨＢｓＡｇ阳性、ＨＢｓＡｇ和ＨＢｅＡｇ双阳性、ＨＢＶＤＮＡ阳性孕妇胎儿ＨＢＶＤＮＡ检出率（５１．３％、９０．２％、８３．８％）均分别显著高于其对照组（３．９％、２１．２％、０．０％）。Ｌｏｇｉｓｔｉｃ回归分析选出的孕妇ＨＢＶ宫内感染危险因素有ＨＢｓＡｇ、ＨＢｅＡｇ、ＨＢＶＤＮＡ和孕期。结论：广西ＨＢＶ宫内感染率较高，ＨＢｓＡｇ阴性孕妇也可能发生ＨＢＶ宫内感染；孕妇ＨＢｓＡｇ、ＨＢｅＡｇ尤其是ＨＢＶＤＮＡ阳性以及孕期是ＨＢＶ宫内感染的主要危险因素。

Objective: To go further into the hepatitis B virus (HBV) intrauterine infection and its risk factors Method: HBV serum markers and HBV DNA were detected by using enzyme linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR) in 116 HBsAg positive and 52 HBsAg negative motherinduced abortion foetuses Results: The positive rates of HBV DNA in the heart blood of foetuses from HBsAg positive pregnant women,HBsAg and HBeAg positve pregnant women and HBV DNA positive pregnant women were 513%,902% and 838%,respectively,which were significantly higher than those (39%,212%,00%) of controlsHBsAgHBeAgHBV DNA and gestational period were selected as risk factors of HBV intrauterine infection by Logistic regression Conclusion: The HBV intrauterine infection rate is rather high in GuangxiHBsAg negative pregnant women may also transmit HBV intrauterine infectionThe main risk factors of HBV intrauterine infection are HBV DNA,HBeAg,gestational period and HBsAg in ORvalue order