慢性阻塞性肺疾病急性发作期痰及血清白介素-8的测定及意义

DETECTION AND SIGNIFICANCE OF INTERLEUKIN8 IN INDUCED SPUTUM AND SERUM FROM ACUTE CHRONIC OBSTRUCTIVE PULMONARY DISEASE

目的：探讨白介素－８（ＩＬ－８）在慢性阻塞性肺疾病（ＣＯＰＤ）气道炎症中的作用。方法：采用酶联免疫吸附法（ＥＬＩＳＡ）检测１０例ＣＯＰＤ急性发作期和１０例正常对照组痰及血清的ＩＬ－８水平。结果：ＣＯＰＤ患者痰ＩＬ－８水平为（１９８２．２４±９０１．０７）ｎｇ／Ｌ、血清ＩＬ－８水平为（６６８．１６±３９８．０５）ｎｇ／Ｌ，明显高于正常人痰（７６９．６±５７０．１４）ｎｇ／Ｌ及血清（１１７．７８±１２３．６１）ｎｇ／Ｌ（Ｐ＜０．０１及Ｐ＜０．０５）。ＣＯＰＤ患者痰ＩＬ－８水平明显高于血清（Ｐ＜０．０１），且无明显相关性（ｒ＝０．１８，Ｐ＞０．０５）。ＣＯＰＤ患者痰ＩＬ－８水平与第一秒用力肺活量百分比（ＦＥＶ１）呈明显负相关（ｒ＝－０．６７，Ｐ＜０．０１）。结论：ＩＬ－８在ＣＯＰＤ气道炎症及气道阻塞中均起重要作用，检测痰ＩＬ－８水平较血清更能反映ＣＯＰＤ气道炎症情况。

Objective: To explore the role of interleukin8(IL8) in the airway inflammation of chronic obstructive pulmonary disease (COPD) Method: IL8 of induced sputum and serum in 10 patients with acute COPD and in 10 normal subjects was detected by ELISA method Results: IL8 of induced sputum (19822490107)ng/L and serum (6681639805)ng/L in patients with cute COPD was much higher than that of induced sputum(769657014)ng/L and serum (1177812361)ng/L in normal subjects (P<001 and P<005)IL8 of induced sputum was also much higher than that of serum in COPD (P<001)And there was no correlation between IL8 of induced sputum and that of serum in patients with COPD (r=018,P>005)In addition,there was high negative correlation between IL8 of induced sputum and FEV1 in patients with COPD (r=-067,P<001) Conclusion: IL8 play an important role in the airway inflammation and airway obstruction of COPDDetection of the level of IL8 in induced sputum was more capable of reflecting airway inflammation of COPD than in serum\=