鼻咽癌及鼻咽部粘膜中EB病毒DNA的检测

DETECTION OF EPSTEIN BARR VIRUS IN NASOPHARYNGEAL CARCINOMA AND NASOPHARYNGEAL MUCOSAS

目的：检测鼻咽部粘膜及鼻咽癌中的ＥＢＶＤＮＡ片段，作为癌前病变的确立和癌变风险的评估指标。方法：用ＰＣＲ扩增ＥＢＶ的ＢａｍＨＩＷ片段。结果：鼻咽癌中阳性率为８７．９％（１０２／１１６），颈淋巴结转移灶６６．６％（２／３），鼻咽癌放疗后鼻咽慢性炎７５．６％（２８／３７），鼻咽粘膜不典型增生７１．１％（１５／２１），鼻咽粘膜慢性炎和正常鼻咽粘膜上皮分别为２１％及２０％。结论：ＥＢＶ感染在鼻咽癌发生过程中起重要作用，并在转移中并不掉失。用ＰＣＲ检测石蜡块中ＥＢＶＤＮＡ，可作为评估癌变和复发风险指标之一。

Objective:Detection of genomic DNA sequence of Epstein Barr virus(EBV) in the nasopharyngeal carcinoma(NPC) and nasopharyngeal mucosas,it may serve as an auxiliary indicators for idenitfying the determination of precancar lesion and risk of canceration Method:Polymerase chain reaction(PCR) was used to amplify the genomic DNA Bam HI W sequence of EBV Results:Positive rate was 87 9%(102/116) in NPC and 66 6%(2/3) in neck metastatic lesions 75 6%(28/37) in chronic nasopharyngitis from NPC patients houing received radiotherapy;71 1%(15/21) in nasopharyngeal epithelium dysplasia;21%(4/19) in chronic nasopharyngitis and 20%(3/15) in non tumor nasopharyngeal epithelium Conclusion:The results show that EBV infection may play an important role in the development and carcinogenesis process of NPC In metastatic process EBV was not lost