地中海贫血高危妊娠胎儿产前绒毛、羊水中巨细胞病毒DNA的检测

DETECTION OF HUMAN CYTOMEGALOVIRUS DNA IN THE PRENATAL CHORIONIC VILLI AND AMNIONIC FLUID OF THE FETUSES WITH THE RISK OF THALASSEMIA MAJOR DURING GESTATION

目的：了解地中海贫血高危妊娠胎儿宫内人巨细胞病毒（ＨＣＭＶ）感染状况。方法：应用聚合酶链反应技术（ＰＣＲ）对４７例地中海贫血高危妊娠胎儿产前绒毛组织、羊水细胞ＤＮＡ进行回顾性人巨细胞病毒ＤＮＡ（ＨＣＭＶ－ＤＮＡ）检测。结果：产前绒毛组织２９例，检出ＨＣＭＶ－ＤＮＡ２例，阳性率为６．９％；羊水细胞１８例，检出ＨＣＭＶ－ＤＮＡ１例，阳性率为５．６％。结论：地中海贫血高危妊娠胎儿与本地区的正常人群一样，产前绒毛、羊水中ＨＣＭＶ感染率较低。

Objective:To investigate HCMV infection states of the fetuses with the risk of thalassemia major during gestation Methods:Polymerase chain reaction (PCR)was used to detect Human Cytomegalovirus DNA (HCMV DNA) in 29 chorionic villi samples and 18 amnionic fliud samples with the risk of thalassemia major Results:2 of the 29 chorionic villi samples and 1 of 18 amnionic fluid samples were positive in HCMV DNA Positive rates were 6 9% and 5 6% Conclusion:HCMV infection rate in the fetuses with the risk of thalassemia major during pregnancy was the same as the normal natives