抑癌候选基因NDRG2对结肠癌细胞SW620的迁移和侵袭力的影响

Effects of Candidate Tumor Suppressor Gene NDRG2 on Migratory and Invasive Ability of Colon Cancer Cells

目的:观察NDRG2对结肠癌SW620细胞侵袭、转移等生物学行为的影响,探讨其可能的调节机制。方法:用阳离子脂质体转染方法分别转染pcDNA3.1-Ndrg2和SiRNA-Ndrg2于SW620细胞内48h,上调/下调NDRG2的表达;检测NDRG2基因mRNA及蛋白表达水平的变化;通过划痕试验及transwell细胞侵袭试验进一步对上调/下调NDRG2表达水平后的结肠癌细胞迁移和侵袭能力进行分析。结果:pcDNA3.1-Ndrg2转染SW620后,NDRG2的mRNA和蛋白表达水平明显升高,细胞的迁移和侵袭能力下降;SiRNA-Ndrg2转染SW620后,NDRG2的mRNA和蛋白表达水平明显降低,细胞的迁移和侵袭能力上升,差异具有统计学意义(P<0.05)。结论:NDRG2作为抑癌候选基因能够降低结肠癌细胞转移和侵袭能力。

Objective： To observe the influence of NDRG2 on the migratory and invasive ability of human colon cancer cell line SW620. Method： pcDNA3.1-Ndrg2 and SiRNA-Ndrg2 were transfected transiently respectively into SW620 by Lipofectamine TM 2000. Reverse transcriptase-polymerase chain reaction （RT-PCR） and Western blot were used to investigate the expression of mRNA and pro- tein of NDRG2. Matrigel invasion assays and wound - healing experiment were used to study the movement and invasion in human colon cancer cell line SW620. Result： After transfecting pcDNA3.1-Ndrg2 into SW620 cells, the mRNA and protein level of NDRG2 increased markedly and the ability of migration and invasion decreased significantly. After transfeting SiRNA-Ndrg2 into SW620 cells, the mRNA and protein level of NDRG2 declined markedly and the ability of migration and invasion increased significantly. Conclusion： NDRG2 as a candidate tumor suppressor gene can reduce the ability of metastasis and invasion of colon cancer cells.