瑞舒伐他汀对载脂蛋白E基因敲除小鼠动脉粥样硬化中调节性T细胞的影响(英文)

Effect of Rosuvastatin on Regulatory T Cell in Atherosclerosis of ApoE KO Mice

目的:探讨瑞舒伐他汀对载脂蛋白E基因敲除(ApoEKO)小鼠动脉粥样硬化中调节性T细胞的影响。方法:首先将30只ApoEKO小鼠建立动脉粥样硬化模型,随机分为高胆固醇饮食组(对照组)、瑞舒伐他汀低剂量组和瑞舒伐他汀高剂量组,各组分别给予蒸馏水或瑞舒伐他汀进行干预8周;将主动脉根部行冰冻切片油红染色,评估粥样硬化斑块面积大小;免疫组织化学法检测主动脉根部粥样硬化斑块处调节性T细胞(Treg)的表达。结果:各组小鼠均有动脉粥样硬化斑块形成,采用瑞舒伐他汀治疗的小鼠动脉粥样硬化斑块的面积明显小于未经治疗的小鼠(P<0.01),同时瑞舒伐他汀能明显增加粥样硬化病变处调节性T细胞的表达,且呈现剂效关系。结论:本实验观察到瑞舒伐他汀不仅能减小ApoEKO小鼠的主动脉粥样硬化斑块,且能使调节性T细胞的表达增多,推测瑞舒伐他汀可以通过促进调节性T细胞的生成而起到抑制动脉粥样硬化的作用。

Objective： To investigate the effect of Rosuvastatin on regulatory T cell in atherosclerosis of Apoe KO mice. Methods： 30 atherosclerotic models of ApoE KO mice were established, then were randomly divided into hypercholesterolemia diet group （control group）, low doses of Rosuvastatin treatment group and high dose group. Each group was interfered with distilled water or Rosuvastatin for 8 weeks, and the aortic roots of the animals were harvested. The atherosclerotic plaque size was detected by oil red staining and the expression of regulatory T cell in atherosclerotic plaque was detected by immunohistochemistry. Results： There were the formation of atherosclerotic plaque in each group of mice; However, the atherosclerotic plaque size in low dose group and high dose group were obvi- ous smaller than that in the untreated mice （P0.01）. Rosuvastatin increased significantly the expression of Treg, which was dose-effect relationship. Conclusion： Rosuvastatin had the effect of promoting the production of regulatory T cell to inhibit atherosclerosis.