甘蓝型油菜芥酸含量的SSR标记分析

Analysis of SSR Markers of Erucic Acid Content in Brassica Napus

目的:探讨高芥酸材料与低芥酸材料杂交效果,为促进高芥酸油菜育种的研究。方法:采用高芥酸材料与低芥酸材料杂交的F2群体作为材料,研究其遗传性状,并对亲本间的芥酸含量进行了SSR标记分析。结果:发现F2群体中的单株芥酸含量受两对基因控制,其遗传规律符合由一对基因控制的分离比例,得到CB10364、Ra2-E12两个共显性标记。结论:CB10364标记与芥酸含量紧密连锁,单株带型为CB10364-a的芥酸含量<6%,单株带型为CB10364-h的芥酸含量6%～36%,带型为CB10364-b的芥酸含量>36%,能较好的区分群体的芥酸含量,该结果可促进高芥酸油菜的育种研究。

Objective： This research was studied to promote the development high erucic acid rape breeding. Method： F2 groups which were hybridized by High erucic acid material and low erucic acid hybrid material were studied here, and the parents＇ erucic acid content was analyzed by SSR markers. Results： The erucic acid content was controlled by two genes, and the inheritance rule accorded with the segregation ratio which is controlled by one pair gene. Meanwhile, two codominant markers of CB10364 and Ra2-E12 were ob- tained. Conclusion： CB10364 markers tightly linked with the erucic acid content. Individual plant strip type of CB10364-a has the erucic acid content 6%, while the type of CB10364-h has the erucic acid content from 6% to 36%, and that of CB10364-b has the erucic acid content 36%. The results can be used on high erucic acid rapeseed breeding, and may be highly availability.